AC voltammetric carbon paste-based enzyme immunosensors
AC voltammetric carbon paste-based enzyme immunosensors
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DOI：
10.1016/s0956-5663(99)00065-2
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发表时间：
2000-02-01
影响因子：
12.6
通讯作者：
Costa-García, A
中科院分区：
文献类型：
--
作者：
Fernández-Sánchez, C;González-García, MB;Costa-García, A
碳糊电极，预先在碱性介质中阳极氧化。是开发新型伏安免疫传感器装置的基础。将适当的免疫化学试剂被动吸附到电极表面上。碱性磷酸酶标记的免疫球蛋白是本工作中使用的示踪剂，3-吲哚酚磷酸是非常适合电化学检测相应亲和反应的酶底物。该分子的水解产生靛蓝二聚体。该产品利用其所表现出的吸附性和固有的电极特性，通过交流伏安法进行检测。在一次测定结束时使用相同的电化学阳极氧化来去除附着在碳糊表面上的整个蛋白质层，从而形成新的传感相并在多个连续实验中使用相同的支撑物。该方法被应用于设计两种不同的免疫测定法来测定人 IgG。电信号具有良好的重现性，检测限约为 10(-10) M。 (C) 2000 Elsevier Science S.A. 保留所有权利。
Carbon paste electrodes, previously anodised in a basic media. are the basis for the development of a new voltammetric immunosensor device. Passive adsorption of the appropriate immunochemical reagent was performed onto the electrode surface. Alkaline Phosphatase labelled immunoglobulin was the tracer used in this work, 3-indoxyl phosphate being a very suitable enzymatic substrate for the electrochemical detection of the corresponding affinity reaction. The hydrolysis of this molecule generates indigo dimmer. This product was detected by alternating current voltammetry taking advantage of the adsorptive and inherent electrodic properties that it exhibits. The same electrochemical anodisation was used at the end of one assay to remove the entire protein layer attached to the carbon paste surface, allowing the formation of a new sensing phase and the use of the same support in several consecutive experiments. The methodology was applied to the design of two different immunoassays for the determination of human IgG. Good reproducibility of the electrodic signal and a limit of detection around 10(-10) M were achieved. (C) 2000 Elsevier Science S.A. All rights reserved.