Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato
Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato
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DOI：
10.1111/j.1365-313x.2005.02365.x
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发表时间：
2005-04-01
期刊：
影响因子：
7.2
通讯作者：
Visser, RGF
中科院分区：
文献类型：
--
作者：
Huang, SW;van der Vossen, EAG;Visser, RGF
比较基因组学提供了一种工具，利用指数增长的序列信息从模式植物克隆农艺学上重要的基因，研究较少的作物物种。植物抗病性A基因座在禾谷类和十字花科的相关物种之间经常缺乏同线性，但在茄科中似乎位置保守。在这份报告中，我们采用了本地RGA的方法，使用基因组信息的模式茄科植物番茄分离R3a，马铃薯基因，赋予种族特异性抗晚疫病病原体致病疫霉。R3a是11号染色体上R3复合体基因座的成员。番茄中R3复合体基因座与相应的l2复合体基因座的比较分析表明，这是一个古老的基因座，参与植物对卵菌和真菌病原体的先天免疫。然而，R3复合物基因座已经从番茄分化后进化，并且该基因座在马铃薯中经历了显著的扩增，而没有破坏侧翼共线性。这种扩张导致了R基因数量的增加和功能的多样化，这可能是由A infestans和其宿主马铃薯之间的共同进化历史所驱动的。观察到R3a基因的组成型表达，以及其功能仍然未知的一些旁系同源物。
Comparative genomics provides a tool to utilize the exponentially increasing sequence information from model plants to clone agronomically important genes from less studied crop species. Plant disease resistance A loci frequently lack synteny between related species of cereals and crucifers but appear to be positionally well conserved in the Solanaceae. In this report, we adopted a local RGA approach using genomic information from the model Solanaceous plant tomato to isolate R3a, a potato gene that confers race-specific resistance to the late blight pathogen Phytophthora infestans. R3a is a member of the R3 complex locus on chromosome 11. Comparative analyses of the R3 complex locus with the corresponding l2 complex locus in tomato suggest that this is an ancient locus involved in plant innate immunity against oomycete and fungal pathogens. However, the R3 complex locus has evolved after divergence from tomato and the locus has experienced a significant expansion in potato without disruption of the flanking colinearity. This expansion has resulted in an increase in the number of R genes and in functional diversification, which has probably been driven by the co-evolutionary history between A infestans and its host potato. Constitutive expression was observed for the R3a gene, as well as some of its paralogues whose functions remain unknown.