Relationship between humoral immunoaugmenting properties of DHEAS and IgD-receptor expression in young and aged mice.
Relationship between humoral immunoaugmenting properties of DHEAS and IgD-receptor expression in young and aged mice.
复制标题
年轻和老年小鼠 DHEAS 体液免疫增强特性与 IgD 受体表达之间的关系。
DOI：
10.1111/j.1749-6632.1995.tb17385.x-i1
复制
发表时间：
1995
影响因子：
5.2
通讯作者：
Thorbecke,GJ
中科院分区：
文献类型：
--
作者：
Swenson,CD;Gottesman,SR;Belsito,DV;Samanich,KM;Edington,J;Thorbecke,GJ
免疫球蛋白D受体与体内抗体产生增加有关，在幼年小鼠中，聚集的免疫球蛋白D可诱导CD4+T细胞产生免疫球蛋白受体，而在老年小鼠中则不能。在目前的研究中，口服或腹腔注射DHEAS被发现增加了抗体的产生，正如空斑形成细胞试验所测量的那样，也导致了年轻和老年小鼠T细胞上IGD-R的表达增加。IGD-R+T细胞用包被IGD的SRBC形成的玫瑰花结细胞计数。如前所述，由于注射单体IGD可阻断IGD-R+T细胞的免疫增强作用，因此在DHEAS处理的小鼠中也检测了单体IGD的作用。在这些研究中，单体IGD的抑制作用表明，DHEAS上调IGD-R在该激素的免疫增强作用中起重要作用。此外，DHEAS对DNCB的接触性超敏反应及幼年和老年小鼠T细胞的增殖反应均无明显影响。经DHEAS治疗后，老年小鼠的Ia+表皮朗格汉斯细胞数量增加，而不是幼年小鼠。
IgD-receptors are associated with augmented antibody production in vivo and are induced on CD4+ T cells by aggregated IgD in young but not in aged mice. In the current study orally or intraperitoneally administered DHEAS was found to enhance antibody production, as measured in a plaque-forming cell assay, and also to cause an increased expression of IgD-R on T cells in both young and aged mice. IgD-R+ T cells are enumerated by rosette cell formation with IgD-coated SRBC. Since, as shown previously, the immunoaugmenting effect of IgD-R+ T cells is blocked by injection of monomeric IgD, the effect of monomeric IgD was also examined in DHEAS-pretreated mice. The inhibitory effect obtained with monomeric IgD in these studies indicates that upregulation of IgD-R by DHEAS plays an important role in the immunoenhancing effect of this hormone. In addition, no significant effect of DHEAS was obtained on contact hypersensitivity to DNCB or on proliferative responses of T cells from young or aged mice. Aged but not young mice showed increases in the numbers of Ia+ epidermal Langerhans cells after DHEAS treatment.