Fructose 1-6 diphosphate prevents intestinal ischemic reperfusion injury and death in rats.
Fructose 1-6 diphosphate prevents intestinal ischemic reperfusion injury and death in rats.
复制标题
果糖 1-6 二磷酸可预防大鼠肠道缺血再灌注损伤和死亡。
DOI：
10.1016/0016-5085(90)91299-l
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发表时间：
1990
期刊：
影响因子：
29.4
通讯作者：
Markov,AK
中科院分区：
文献类型：
--
作者：
Sun,JX;Farias,LA;Markov,AK
这项对大鼠缺血和缺血后再灌注肠损伤的研究评估了果糖1-6二磷酸的潜在治疗价值，其基础是其在缺血期间增强无氧碳水化合物代谢的能力，以及通过抑制中性粒细胞产生氧自由基来防止重建血流后的额外组织损伤。为了实现这一目标，将28只大鼠随机分为4组：1-6二磷酸果糖预处理组（n = 7）;葡萄糖预处理组（n = 7）; 1-6二磷酸果糖再灌注后处理组（n = 7）;和生理盐水再灌注后处理组（n = 7）。另外5只大鼠进行假手术。在上级肠系膜动脉闭塞30分钟后，所有大鼠接受其各自的治疗5天。对照组大鼠再灌注后动脉压显著降低（p < 0.001），与1-6-二磷酸果糖组相比也是如此（p < 0.001）。对照组的白色细胞计数显著增加（p < 0.001），而1-6二磷酸果糖组的白色细胞计数与缺血前值无差异。所有在< 5天内死亡的对照组大鼠均出现透壁性肠坏死，而在存活5天的对照组大鼠中，有3只出现部分肠坏死。只有一个果糖1-6二磷酸治疗的大鼠有部分肠坏死。假手术组大鼠的5天总存活率为100%，1-6二磷酸果糖治疗组大鼠为93%，对照组为21%（1-6二磷酸果糖vs.对照组，p < 0.001; 1-6二磷酸果糖vs.假手术组，NS）。根据果糖1-6二磷酸的药理学性质，对结果进行了讨论和解释。
This study of ischemic and postischemic reperfusion intestinal injury in rats evaluates the potential therapeutic value of fructose 1–6 diphosphate on the basis of its ability to enhance anaerobic carbohydrate metabolism during ischemia and to prevent additional tissue injury after reestablishing blood flow by inhibiting the neutrophils to produce oxygen free radicals. In pursuit of this goal, 28 rats were randomized into 4 groups: pretreated with fructose 1–6 diphosphate (n = 7); pretreated with glucose (n = 7); postreperfusion treated with fructose 1–6 diphosphate (n = 7); and postreperfusion treated with saline (n = 7). Five additional rats were sham operated. Following 30 min occlusion of the superior mesenteric artery, all rats received their respective treatments for 5 days. Postreperfusion arterial pressure was significantly lower in the control rats (p < 0.001) as well as when compared with the fructose 1–6 diphosphate groups (p < 0.001). Significant increase in white blood cell counts occurred in the controls (p < 0.001), whereas in the fructose 1–6 diphosphate groups white blood cell counts were no different from preischemic values. All control rats that died in < 5 days had transmural intestinal necrosis, whereas in 3 of the controls that survived 5 days, partial intestinal necrosis was noted. Only one fructose 1–6 diphosphate-treated rat had partial intestinal necrosis. The overall 5-day survival was 100% for shamoperated rats, 93% for fructose 1–6 diphosphatetreated rats, and 21% for controls (fructose 1–6 diphosphate vs. controls, p < 0.001; fructose 1–6 diphosphate vs. sham, NS). The results are discussed and explained in terms of the postulated mechanism based on the pharmacological properties of fructose 1–6 diphosphate.