SIMPLE, RAPID, AND EFFECTIVE METHOD OF PRODUCING AORTOCAVAL SHUNTS IN THE RAT
SIMPLE, RAPID, AND EFFECTIVE METHOD OF PRODUCING AORTOCAVAL SHUNTS IN THE RAT
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DOI：
10.1093/cvr/24.5.430
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发表时间：
1990-05-01
影响因子：
10.8
通讯作者：
DIEBOLD, S
中科院分区：
文献类型：
--
作者：
GARCIA, R;DIEBOLD, S
该研究的目的是开发一种新的方法，在不进行血管显微手术的情况下，在大鼠身上产生皱胃腹主动脉-腔静脉分流术。剖腹手术显露下腔静脉和腹主动脉。用18号一次性穿刺针将主动脉穿入左肾动脉尾侧，穿透主动脉与下腔静脉之间的相邻壁面，穿透左肾动脉。将牛头犬血管夹放在穿刺点的头部，拔出针，用一滴氰基丙烯酸酯胶封住主动脉穿刺点。30岁后，S将夹子取下。通过腔静脉肿胀和动静脉血液的混合，从视觉上证实了分流的通畅。未见局部出血。手术结束后，手术结束了。这一过程只需不到10分钟。11只接受此手术的大鼠中，仅1只在24小时内死亡，其余动物在手术后4周全部处死。在这10只动物中，有9只出现了大致相同规模的心肌肥大。假操作的对照组没有变化。这是一种可重复、简单、快速的大鼠高输出量心力衰竭和心肌肥厚的方法，可在许多实验室使用。
The aim of the study was to develop a new procedure to produce abominal aortocaval shunts in the rat without vascular microsurgery. The inferior vena cava and abdominal aorta were exposed by lapartomy. The aorta was punctured caudal to the left renal artery with an 18 gauge disposable needle which was advanced into the vessel, perforating the adjacent wall between aorta and vena cava and penetrating the latter. A bulldog vascular clamp was placed across the aorta cephalic to the puncture, the needle was withdrawn, and the aortic puncture point was sealed with a drop of cyanoacrylate glue. The clamp was removed 30 s later. Patency of the shunt was verified visually by swelling of the vena cava and admixture of arterial and venous blood. No local haemorrhages were seen. The laporatomy was then closed. The procedure takes less than 10 min. Of 11 rats which received this procedure, only one died within 24 h. All the other animals were killed 4 weeks after operation. Nine of these 10 animals had developed cardiac hypertrophy of about the same magnitude. There were no changes in sham operated controls. This is a reproducible, simle and rapid method of developing high output heart failure and cardiac hypertrophy in the rat which could be useful in many laboratories.