Single-Cell Analysis of Somatotopic Map Formation in the Zebrafish Lateral Line System
Single-Cell Analysis of Somatotopic Map Formation in the Zebrafish Lateral Line System
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DOI：
10.1002/dvdy.22324
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发表时间：
2010-07-01
影响因子：
2.5
通讯作者：
Takeda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Sato, Akira;Koshida, Sumito;Takeda, Hiroyuki
斑马鱼的侧线是一个简单的感觉系统，除了它们的感官--神经肥大，还有少量的神经元。我们采用这一系统作为地形图形成的单细胞水平分析的模型，并研究了何时以及如何建立侧线地形图。单神经元标记显示，在受精后54小时，神经节出现了体位组织，但这一初始地图并不像受精后6天观察到的那样准确。在这个初始阶段，单个神经元表现出非常不同的行为和形态。我们确定了先导神经元，其轴突最先到达尾部，以及后来出现的轴突，它们对最初的地图做出了贡献。我们的数据表明，从侧线发育开始，侧线神经元就是异质性的，其中一些神经元天生就被命运决定了对躯体定位图的贡献。发展动力学239：2058-2065,2010。(C)2010年Wiley-Liss公司
The zebrafish lateral line is a simple sensory system comprising a small number of neurons in addition to their sensory organs, the neuromasts. We have adopted this system as a model for single-cell level analyses of topographic map formation and examined when and how the lateral line topographic map is established. Single-neuron labeling demonstrated that somatotopic organization of the ganglion emerges by 54 hr postfertilization, but also that this initial map is not as accurate as that observed at 6 days postfertilization. During this initial stage, individual neurons exhibit extensively diverse behavior and morphologies. We identified leader neurons, the axons of which are the first to reach the tail, and later-appearing axons that contribute to the initial map. Our data suggest that lateral line neurons are heterogeneous from the beginning of lateral line development, and that some of them are intrinsically fate determined to contribute to the somatotopic map. Developmental Dynamics 239:2058-2065, 2010. (C) 2010 Wiley-Liss, Inc.