Epidermal growth factor in the submandibular glands of inbred mice.
Epidermal growth factor in the submandibular glands of inbred mice.
复制标题
近交系小鼠颌下腺中的表皮生长因子。
DOI：
10.1002/aja.1001600305
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
Barka,T
中科院分区：
文献类型：
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作者：
Tom-Moy,M;Barka,T
表皮生长因子(EGF)是一种雄激素依赖性多肽，在雄性小鼠颌下腺中含量较高。用放射免疫法测定了6个近交系(129/J、C57BL/6J、C58/J、SWR/J、RF/J、A/J)成年雄性和雌性小鼠的腺体EGF。在所有品系中，雄性的腺体含有的表皮生长因子是雌性的30到500倍。此外，不同品系间EGF含量存在显著的性别差异，Rf/J品系EGF含量最高，C57BL/6J品系EGF含量最低，两品系雄性EGF含量为3只，雌性EGF含量为4只，对影响EGF含量的因素进行了进一步的分析。在21日龄时，两个品系的小鼠的腺体中都可以检测到EGF，此后迅速增加，直到14周龄。在整个出生后发育过程中，RF/J小鼠的腺体中的EGF水平高于C57BL/6J小鼠。去势后30d，两个品系的EGF水平下降了约98%，但品系差异并未被消除。在去势的小鼠体内植入睾酮(硅胶管中1毫克)，与去势的小鼠相比，其EGF水平是前者的6到10倍。即使在血浆睾酮水平相似的诱导动物中，通过放射免疫测定，两种菌株之间的EGF水平也明显不同。然而，在每天服用5-α-双氢睾酮3-14天后，并没有看到这样的差异。EGF免疫细胞化学染色显示，RF/J小鼠腺体中的EGF多肽浓度高于C57BL/6J小鼠，并证实EGF仅定位于颗粒曲管(GCT)细胞。根据我们的形态计量学分析，在雄性RF/J小鼠的腺体中，GCT区占腺体体积的比例比C57BL/6J小鼠大8%(P<；0.001)。然而，两个品系雌性小鼠的腺体相对GCT体积的差异在统计学上并不显著。EGF的含量与GCTS的相对体积之间无直接相关性。所获得的证据表明，颌下腺EGF水平的菌株差异是由遗传决定的。
Epidermal growth factor (EGF), an androgen‐dependent polypeptide, occurs in high concentration in male mouse submandibular gland. Glands of adult male and female mice of six inbred strains (129/J, C57BL/6J, C58/J, SWR/J, RF/J, A/J) were assayed for EGF by radioimmunoassay. In all strains, the glands of males contained 30 to 500‐fold more EGF than those of females. Furthermore, significant differences in EGF content were found among the various strains in both sexes; the highest amount of EGF was present in RF/J and the lowest in C57BL/6J, with a ratio of three in the males and four in the females of the two strains, respectively.Factors that effect EGF levels were analyzed further, using these two strains. EGF was measurable in the glands of mice of both strains at 21 days of age and increased rapidly thereafter, up to 14 weeks of age. Throughout postnatal development, the level of EGF was greater in the glands of RF/J mice than in those of the C57BL/6J animals. Thirty days after castration, the EGF levels were reduced by about 98% in both strains, but the strain difference was not abolished. Testosterone implants (1 mg in Silastic tube) in castrated mice induced EGF levels six‐ to ten‐fold compared to castrates. Even in induced animals, which had similar plasma testosterone levels, as measured by radioimmunoassays, the difference in EGF levels between the two strains was manifest. Such a difference, however, was not seen after the daily administration of 5‐alpha‐dihydrotestosterone for 3–14 days. Immunocytochemical staining for EGF also indicated a higher concentration of the polypeptide in the glands of RF/J mice than in those of C57BL/6J animals, and confirmed the exclusive localization of EGF in the cells of the granular convoluted tubules (GCT). According to our morphometric analysis, in the glands of male RF/J mice the GCT compartment occupied a greater portion (8% greater, P<0.001) of the gland volume than in C57BL/6J mice. The difference in the relative GCT volumes in the glands of female mice of the two strains was, however, statistically not significant. There was no direct correlation between the amount of EGF and the relative volume of the GCTs in the two strains. The evidence obtained implies that strain differences in submandibular‐gland EGF levels are determined genetically.