Experimental assessment of electromigration properties of background electrolytes in capillary zone electrophoresis
Experimental assessment of electromigration properties of background electrolytes in capillary zone electrophoresis
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DOI：
10.1002/elps.200305539
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发表时间：
2004-01-01
期刊：
影响因子：
2.9
通讯作者：
Bocek, P
中科院分区：
文献类型：
--
作者：
Bousková, E;Presutti, C;Bocek, P
毛细管区带电泳（CZE）中背景电解质的电迁移分散（EMD）特性对分析的成功与否至关重要。这些性质的知识可以很好地用于预测分析物的峰的不对称性，用于预测可能预期系统区的强干扰的不安全区域，以及用于选择感兴趣的分析物可能给出尖锐且实际上对称的峰的最佳条件。目前的理论使人们能够计算和预测许多BGE的EIVID属性，但也有很多BGE超出了目前的理论模型，只要他们的组成和平衡所涉及的考虑。这一贡献带来了一种方法，从容易获得的实验数据的EMD性能的任何BGE的评估。所提出的方法说明了模型的例子，阳离子和阴离子分离。咪唑乙酸盐、组胺乙酸盐和组氨酸乙酸盐作为阳离子分离的模型BGE;作为阴离子分离的模型BGE，选择了Tris-硼酸盐和硼酸钠BGE，因为这些缓冲液经常使用，硼酸盐因其在水溶液中的络合平衡而闻名。
Electromigration dispersion (EMD) properties of background electrolytes (BGEs) used in capillary zone electrophoresis (CZE) are of key importance for the success of an analysis. The knowledge of these properties may serve well for the prediction of the asymmetry of peaks of analytes, for the prediction of unsafe regions where a strong interference of system zones may be expected, and for the selection of optimum conditions where the analytes of interest may give sharp and practically symmetric peaks. Present theories enable one to calculate and predict EIVID properties of many BGEs but there is also a lot of BGEs that are beyond the present theoretical models as far as their composition and equilibria involved are considered. This contribution brings a method for assessment of EMD properties of any BGE from easily accessible experimental data. The method proposed is illustrated by model examples both for cationic and anionic separations. Imidazole acetate, histamine acetate, and histidine acetate served as model BGEs for cationic separations; as the model BGE for anionic separations, Tris-borate and sodium-borate BGEs have been selected since these buffers are frequently used and borate is well-known for its complexing equilibria in aqueous solutions.