The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2).
The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2).
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PPGPP合成在形态分化和抗生素产生中的全球作用在Coelicolor A3（2）中。
DOI：
10.1186/gb-2007-8-8-r161
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发表时间：
2007
期刊：
影响因子：
12.3
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中科院分区：
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天蓝色链霉菌中ppGpp合成的诱导影响了链霉菌生物学特征的几个基因组元素的表达，包括抗生素基因簇、保守子和形态发生蛋白。在许多细菌中，通过严格因子ppGpp响应氨基酸饥饿来调节翻译装置的产生是保守的。然而，除了这一核心功能，很明显，ppGpp还表现出属特异性调节作用。在这项研究中，我们使用Affyssin基因芯片更全面地表征ppGpp合成对天蓝色链霉菌A3生物学的调节影响（2），重点是抗生素生物合成和形态分化的控制。ppGpp合成的诱导抑制了主要sigma因子hrdB的转录，这些基因具有与活跃生长相关的功能，并且S.腔棘鱼基因组ppGpp合成后诱导的基因包括替代σ因子SCO 4005，许多用于产生抗生素CDA和放线菌紫素，调节基因SCO 4198和SCO 4336，以及两种替代核糖体蛋白。CDA和放线菌紫素簇的诱导分别伴随着通路调节剂cdaR和actII-ORF 4的转录增加。比较转录组档案的relA空菌株，M570，不能ppGpp合成与其亲本M600表明发生代谢应激的突变体。M570未能形成孢子与表面活性肽SapB和疏水蛋白的产生之间的停滞有关：它过量产生SapB，但未能表达卓别林和rodlin基因。In S. coelicolor，ppGpp合成影响链霉菌生物学特征的几个基因组元件的表达，特别是抗生素基因簇、保守子和形态发生蛋白。
The induction of ppGpp synthesis in Streptomyces coelicolor influenced the expression of several genomic elements characteristic of streptomycete biology, including antibiotic gene clusters, conservons, and morphogenetic proteins. Regulation of production of the translational apparatus via the stringent factor ppGpp in response to amino acid starvation is conserved in many bacteria. However, in addition to this core function, it is clear that ppGpp also exhibits genus-specific regulatory effects. In this study we used Affymetrix GeneChips to more fully characterize the regulatory influence of ppGpp synthesis on the biology of Streptomyces coelicolor A3(2), with emphasis on the control of antibiotic biosynthesis and morphological differentiation. Induction of ppGpp synthesis repressed transcription of the major sigma factor hrdB, genes with functions associated with active growth, and six of the thirteen conservons present in the S. coelicolor genome. Genes induced following ppGpp synthesis included the alternative sigma factor SCO4005, many for production of the antibiotics CDA and actinorhodin, the regulatory genes SCO4198 and SCO4336, and two alternative ribosomal proteins. Induction of the CDA and actinorhodin clusters was accompanied by an increase in transcription of the pathway regulators cdaR and actII-ORF4, respectively. Comparison of transcriptome profiles of a relA null strain, M570, incapable of ppGpp synthesis with its parent M600 suggested the occurrence of metabolic stress in the mutant. The failure of M570 to sporulate was associated with a stalling between production of the surfactant peptide SapB, and of the hydrophobins: it overproduced SapB but failed to express the chaplin and rodlin genes. In S. coelicolor, ppGpp synthesis influences the expression of several genomic elements that are particularly characteristic of streptomycete biology, notably antibiotic gene clusters, conservons, and morphogenetic proteins.