Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves
Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves
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DOI：
10.1104/pp.105.059907
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发表时间：
2005-07-01
期刊：
影响因子：
7.4
通讯作者：
Triantaphylidès, C
中科院分区：
文献类型：
--
作者：
Montillet, JL;Chamnongpol, S;Triantaphylidès, C
我们最初比较了不同细胞死亡事件期间烟草（Nicotiana tabacum）叶子中的脂质过氧化谱。上游氧脂素测定用于区分活性氧 (ROS) 介导的脂质过氧化与 9-和 13-脂加氧酶 (LOX) 依赖性脂质过氧化。在过氧化氢酶缺陷植物叶子中 H2O2 依赖性细胞死亡过程中，测量了自由基介导的膜过氧化。利用这些转基因植物，我们证明，在光照条件下，H2O2 在执行由激发子 Cryptogein 触发的细胞死亡中发挥着重要作用，它会引发类似的 ROS 介导的脂质过氧化。然而，在黑暗条件下，隐花素诱导的细胞死亡不依赖于 H2O2，并且伴随着 9-LOX 途径的产物。在无毒病原体丁香假单胞菌丁香致病变种诱导的过敏反应中，9-LOX 和氧化过程同时进行，其中 ROS 介导的脂质过氧化在光照下占主导地位。因此，我们的结果证明了 H2O2 和脂质氢过氧化物之间的紧密相互作用，并强调了光在过敏反应过程中的重要性。
We initially compared lipid peroxidation profiles in tobacco (Nicotiana tabacum) leaves during different cell death events. An upstream oxylipin assay was used to discriminate reactive oxygen species (ROS)- mediated lipid peroxidation from 9- and 13-lipoxygenase (LOX)-dependent lipid peroxidation. Free radical-mediated membrane peroxidation was measured during H2O2-dependent cell death in leaves of catalase-deficient plants. Taking advantage of these transgenic plants, we demonstrate that, under light conditions, H2O2 plays an essential role in the execution of cell death triggered by an elicitor, cryptogein, which provokes a similar ROS-mediated lipid peroxidation. Under dark conditions, however, cell death induction by cryptogein was independent of H2O2 and accompanied by products of the 9-LOX pathway. In the hypersensitive response induced by the avirulent pathogen Pseudomonas syringae pv syringae, both 9-LOX and oxidative processes operated concurrently, with ROS-mediated lipid peroxidation prevailing in the light. Our results demonstrate, therefore, the tight interplay between H2O2 and lipid hydroperoxides and underscore the importance of light during the hypersensitive response.