Kinetic analysis of the specific host response to a murine gammaherpesvirus.
Kinetic analysis of the specific host response to a murine gammaherpesvirus.
复制标题
对鼠伽马疱疹病毒的特定宿主反应的动力学分析。
DOI：
10.1128/jvi.72.2.943-949.1998
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发表时间：
1998
影响因子：
5.4
通讯作者：
Doherty,PC
中科院分区：
文献类型：
--
作者：
Stevenson,PG;Doherty,PC
BALB/c 小鼠呼吸道感染鼠伽玛疱疹病毒 68 (MHV-68) 会诱导区域纵隔淋巴结 (MLN) 中病毒特异性细胞毒性 T 淋巴细胞 (CTL) 前体 (CTLp) 的克隆扩增。其中一些 CTL 分化成为功能齐全的 CTL 效应器，可以在淋巴组织和肺部病理部位中检测到。尽管淋巴结和脾脏终生含有大量潜伏感染的 B 细胞，但所有部位的病毒特异性 CTL 活性水平都迅速下降。在呼吸道上皮中 MHV-68 感染的生产期终止后的几个月内，MLN 中的 CD8+CTLp 数量降至背景水平，但在脾脏中保持在相对较低的频率。在培养的脾细胞中也可以证明产生 γ 干扰素的 MHV-68 特异性 CD4+ 组的持续存在。病毒特异性免疫球蛋白 G (IgG) 反应发展缓慢，血清中和抗体和酶联免疫吸附测定滴度持续几个月持续上升。感染后 2 周内，血清总 IgG 水平急剧升高，可能是多克隆 B 细胞激活的结果，并且仍然很高。免疫反应特征明显受到这种 DNA 病毒持续存在的影响。
Respiratory infection of BALB/c mice with the murine gammaherpesvirus 68 (MHV-68) induces the clonal expansion of virus-specific cytotoxic T-lymphocyte (CTL) precursors (CTLp) in the regional, mediastinal lymph nodes (MLN). Some of these CTLps differentiate to become fully functional CTL effectors, which can be detected in both the lymphoid tissue and in the site of pathology in the lung. Though the lymph nodes and spleen harbor substantial populations of latently infected B cells for life, the level of virus-specific CTL activity decreases rapidly in all sites. The CD8+CTLp numbers fall to background levels in the MLN within several months of the termination of the productive phase of MHV-68 infection in the respiratory epithelium but are maintained at relatively low frequency in the spleen. The continued presence of a gamma interferon-producing, MHV-68-specific CD4+set can also be demonstrated in cultured spleen cells. The virus-specific immunoglobulin G (IgG) response is slow to develop, with serum neutralizing antibody and enzyme-linked immunosorbent assay titers continuing to rise for several months. The level of total serum IgG increases dramatically within 2 weeks of infection, probably as a consequence of polyclonal B-cell activation, and remains high. The immune response profile is clearly influenced by the persistence of this DNA virus.