The complete genome and proteome of Mycoplasma mobile
The complete genome and proteome of Mycoplasma mobile
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DOI：
10.1101/gr.2674004
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发表时间：
2004-08-01
期刊：
影响因子：
7
通讯作者：
Church, GM
中科院分区：
文献类型：
--
作者：
Jaffe, JD;Stange-Thomann, N;Church, GM
虽然通常被认为是“最小”的生物体，但支原体在宿主环境、表型性状和致病性方面表现出广泛的多样性。在这里，我们报告了完整的基因组序列和蛋白质基因组图的鱼支原体移动支原体，注意到其强大的滑行运动。蛋白质组学数据首次用于新基因组的主要注释，为许多预测蛋白质的表达提供了验证。研究人员发现了几个新的特征，包括在五个完整的拷贝中存在一个类似于2435的DNA重复单元，这些重复单元被证明编码几乎相同但唯一表达的蛋白质。M. mobile是目前报道的生物中DNA GC含量最低（24.9%）和trna减少最多的生物之一（28）。在基因组中，串联复制和横向基因转移的许多实例都是显而易见的。其他运动和不运动支原体的多个可用的完整基因组序列使我们能够使用比较基因组学和系统发育方法来提出可能参与运动的几个候选基因。这些分析的结果表明，在支原体谱系中，滑行运动可能不止一次独立出现。
Although often considered "minimal" organisms, mycoplasmas show a wide range of diversity with respect to host environment, phenotypic traits, and pathogenicity. Here we report the complete genomic sequence and proteogenomic map for the piscine mycoplasma Mycoplasma mobile, noted for its robust gliding motility. For the first time, proteomic data are used in the primary annotation of a new genome, providing validation of expression for many of the predicted proteins. Several novel features were discovered including along repeating unit of DNA of similar to2435 by present in five complete copies that are shown to code for nearly identical yet uniquely expressed proteins. M. mobile has among the lowest DNA GC contents (24.9%) and most reduced set of tRNAs of any organism yet reported (28). Numerous instances of tandem duplication as well as lateral gene transfer are evident in the genome. The multiple available complete genome sequences for other motile and immotile mycoplasmas enabled us to use comparative genomic and phylogenetic methods to suggest several candidate genes that might be involved in motility. The results of these analyses leave open the possibility that gliding motility might have arisen independently more than once in the mycoplasma lineage.