Dietary phytoestrogens improve stroke outcome after transient focal cerebral ischemia in rats
Dietary phytoestrogens improve stroke outcome after transient focal cerebral ischemia in rats
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DOI：
10.1111/j.1460-9568.2006.04599.x
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发表时间：
2006-02-01
影响因子：
3.4
通讯作者：
Alborch, E
中科院分区：
文献类型：
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作者：
Burguete, MC;Torregrosa, G;Alborch, E
由于植物雌激素被认为是神经保护剂，我们评估了饮食中的异黄酮型植物雌激素对缺血性中风具有神经保护作用的假设。采用线栓法制备大鼠大脑中动脉闭塞(MCAO)模型，在饲喂大豆饲料和不含异黄酮饲料的大鼠上复制短暂性局灶性脑缺血(90min)模型。分别于术前、术中、术后监测大脑皮质激光多普勒血流(皮质血流灌注，CP)、动脉血压、中心体温、PaO2、PaCO2、pH和血糖。3天后进行神经功能检查和脑梗塞体积测量。用高效液相色谱法测定饲料中异黄酮苷和苷元的含量。大豆饮食组和无异黄酮组的缺血前、缺血内和缺血后的CP值均无显著差异。用大豆饲料喂养的动物显示，脑梗塞体积为122+/-20.2 mm(3)(占整个同侧大脑半球体积的19+/-3.3%)。饲喂无异黄酮组动物的平均脑梗塞体积显著增加，为191+/-26.7 mm(28+/-4.1%，P<0.05)。神经学检查显示，无异黄酮组的功能障碍明显高于豆类饮食组(3.3+/-0.5vs.1.9+/-0.5，P<；0.05)。这些结果表明，饮食异黄酮能改善短暂性局灶性脑缺血后的卒中预后，其机制是饮食中的异黄酮量越高，脑梗塞体积越小，神经状态越好。
As phytoestrogens are postulated as being neuroprotectants, we assessed the hypothesis that dietary isoflavone-type phytoestrogens are neuroprotective against ischemic stroke. Transient focal cerebral ischemia (90 min) was induced by middle cerebral artery occlusion (MCAO) following the intraluminal thread technique, both in rats fed with soy-based diet and in rats fed with isoflavone-free diet. Cerebro-cortical laser-Doppler flow (cortical perfusion, CP), arterial blood pressure, core temperature, PaO2, PaCO2, pH and glycemia were measured before, during and after MCAO. Neurological examination and infarct volume measurements were carried out 3 days after the ischemic insult. Dietary isoflavones (both glycosides and aglycones) were measured by high-performance liquide chromatography. Neither pre-ischemic, intra-ischemic nor post-ischemic CP values were significantly different between the soy-based diet and the isoflavone-free diet groups. Animals fed with the soy-based diet showed an infarct volume of 122 +/- 20.2 mm(3) (19 +/- 3.3% of the whole ipsilateral hemisphere volume). In animals fed with the isoflavone-free diet the mean infarct volume was significantly higher, 191 +/- 26.7 mm(3) (28 +/- 4.1%, P < 0.05). Neurological examination revealed significantly higher impairment in the isoflavone-free diet group compared with the soy-based diet group (3.3 +/- 0.5 vs. 1.9 +/- 0.5, P < 0.05). These results demonstrate that dietary isoflavones improve stroke outcome after transient focal cerebral ischemia in such a way that a higher dietary isoflavone content results in a lower infarct volume and a better neurological status.