Non-contact micromanipulation system with computer vision
Non-contact micromanipulation system with computer vision
复制标题
计算机视觉非接触式显微操作系统
DOI：
10.1007/978-3-540-89208-3_576
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
H. Kitajima
中科院分区：
文献类型：
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作者：
Y. Tanaka;H. Kawada;K. Hirano;M. Ishikawa;H. Kitajima
Ashkin在1970年首次展示的激光捕获是一种功能强大的微或纳米操作工具，适用于封闭的工作空间，如芯片实验室，因为它能够在没有物理接触的情况下操作微米尺寸的物体，与使用玻璃毛细管的机械微操作器相反。然而，如果意图是灵巧地或自动地实时操纵对象，则常规激光捕获系统的控制和视觉系统不足以在三维空间中操纵对象。我们开发了一个具有良好的用户界面和实时图像处理功能的光镊系统。本文提出了一种基于视觉信息的自动化非接触微操作的概念及其适用领域，并描述了所开发系统的系统结构。几个示范，即微珠和微米级的非球形物体的自动化安排的结果，也被描述。在我们的自动化操作实验中，分散在水中的微珠通过实时图像处理技术检测，然后捕获并运输以按所需图案排列。在其他实验中，晶须和硅藻的非球形形状悬浮在水中的自动检测和稳定的多个捕获光束的同时照射捕获。
Laser trapping, which was first demonstrated in 1970 by Ashkin and is well-known as optical tweezers, is a powerful micro- or nano-manipulation tool suitable for a closed working space such as a Lab-on-a-Chip, since it enables the manipulation of micron-sized objects without physical contact, in contrast to a mechanical micro-manipulator using a glass capillary. However, the control and vision systems of a conventional laser trapping system are insufficient to manipulate the objects in 3-dimensional space, if the intention is to dexterously or automatically manipulate the objects in real-time. We have developed an optical tweezers system with an excellent user-interface and real-time image processing functions. In the present paper, a concept of automated noncontact micromanipulation based on visual information and fields suitable for its application are proposed, and the system configuration of the developed system is described. The results of several demonstrations, namely the automated arrangement of micro-beads and micron-sized non-spherical objects, are also described. In our automated manipulation experiment, the micro-beads dispersed in water are detected by a real-time image processing technique, and then trapped and transported to be arranged in desired patterns. In the other experiments, whiskers and diatoms of non-spherical shape suspended in water are automatically detected and stably trapped by the simultaneous irradiation with multiple trapping beams.