Molecular cloning and characterization of a new insulin/IGF-like peptide of the nematode Caenorhabditis elegans
Molecular cloning and characterization of a new insulin/IGF-like peptide of the nematode Caenorhabditis elegans
复制标题
DOI：
10.1006/bbrc.2000.2971
复制
发表时间：
2000-07-05
影响因子：
3.1
通讯作者：
Kimura, Y
中科院分区：
文献类型：
--
作者：
Kawano, T;Ito, Y;Kimura, Y
秀丽隐杆线虫的滞育、衰老和脂肪积累受哺乳动物胰岛素/胰岛素样生长因子-I（IGF-I）受体的同系物β-2调节。我们克隆并鉴定了一个C. elegans基因编码一种新的胰岛素/IGF样肽。该基因含有三个外显子，编码一个95个残基长的前体蛋白。虽然推定的前体含有信号肽、B链、C肽和A链，如前胰岛素原，但成熟肽由一种多肽组成，如IGF。预测的三级结构似乎与胰岛素的晶体结构相似。因此，该肽可能是胰岛素和IGF的杂合分子。在胚胎和几个幼虫阶段检测到肽的表达。肽生产的中断导致寿命延长，就像daf-2突变一样，这表明肽应该是DAF-2的配体之一。这是第一次描述的肽，介导动物寿命。(C)北京大学出版社.
Diapause, aging, and fat accumulation in Caenorhabditis elegans are regulated by DAF-2, a homolog of mammalian insulin/insulin-like growth factor-I (IGF-I) receptors. We have cloned and characterized a C. elegans gene encoding a new insulin/IGF-like peptide. The gene containing three exons encodes a precursor protein 95 residue long. Although the putative precursor contains a signal peptide, B chain, C peptide, and A chain like the preproinsulin, the mature peptide consists of one polypeptide-like IGF. The predicted tertiary structure seems similar to crystal structure of insulin. Therefore, the peptide may be a hybrid molecule of insulin and IGF. The peptide expression was detected at the embryonic and several larval stages. Disruption of the peptide production led to an extended life span like the daf-2 mutation, suggesting that the peptide should be one of the ligands of the DAF-2. This is the first description of the peptide that mediates animal longevity. (C) 2000 Academic Press.