Different subcellular distributions of the vesicular monoamine transporter, VMAT2, in subclasses of sympathetic neurons.
Different subcellular distributions of the vesicular monoamine transporter, VMAT2, in subclasses of sympathetic neurons.
复制标题
囊泡单胺转运蛋白 VMAT2 在交感神经元亚类中的不同亚细胞分布。
DOI：
10.1016/j.brainres.2006.10.073
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发表时间：
2007
期刊：
影响因子：
2.9
通讯作者：
Horn,JohnP
中科院分区：
文献类型：
--
作者：
Headley,DrewB;Suhan,NadineM;Horn,JohnP
大鼠颈上神经节 (SCG) 中的一个神经元亚群被发现缺乏 VMAT2 的免疫染色，VMAT2 是一种囊泡单胺转运蛋白的异构体，可将儿茶酚胺装载到囊泡中并在突触处释放。神经肽 Y (NPY)（血管运动神经元的标记物）的双重标记揭示了 NPY 的选择性细胞共定位以及 VMAT2 的强烈核周染色。这意味着 VMAT2 阴性神经元可能具有分泌运动和立毛运动表型。我们通过酪氨酸羟化酶 (TH) 免疫反应性的表达来识别外周去甲肾上腺素能轴突，并确定它们是否也表达 NPY 和 VMAT2，从而对此进行了测试。该分析揭示了下颌下腺和竖毛毛基部存在 VMAT2 阳性、非血管舒缩交感神经轴突。这些结果共同证实了早期的迹象，即大鼠 SCG 中几乎所有交感神经元都表达 VMAT2，并且它们首次表明细胞的功能亚类可以通过 VMAT2 的不同体细胞免疫反应水平来区分。
A subpopulation of neurons in the rat superior cervical ganglion (SCG) was found to lack immunostaining for VMAT2, an isoform of the vesicular monoamine transporter that loads catecholamines into vesicles for release at the synapse. Double labeling with neuropeptide Y (NPY), a marker for vasomotor neurons, revealed selective cellular colocalization of NPY together with intense perinuclear staining for VMAT2. This implied that VMAT2-negative neurons were likely to have secretomotor and pilomotor phenotypes. We tested this by identifying peripheral noradrenergic axons by their expression of immunoreactivity for tyrosine hydroxylase (TH) and determining whether they also expressed NPY and VMAT2. This analysis revealed the presence of VMAT2-positive, non-vasomotor sympathetic axons in the submandibular gland and at the base of piloerector hairs. Together the results confirm earlier indications that virtually all sympathetic neurons in the rat SCG express VMAT2 and they show for the first time that functional subclasses of cells can be distinguished by different somatic levels of immunoreactivity for VMAT2.