Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network.
Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network.
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比较磷酸化蛋白质组学揭示 MKK2 在香蕉（Musa spp.）冷信号网络中的重要作用
DOI：
10.1038/srep40852
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发表时间：
2017-01-20
影响因子：
4.6
通讯作者：
Yang QS
中科院分区：
文献类型：
--
作者：
Gao J;Zhang S;He WD;Shao XH;Li CY;Wei YR;Deng GM;Kuang RB;Hu CH;Yi GJ;Yang QS
低温是影响全球香蕉生产的主要环境胁迫之一。然而，很少有人知道的全球磷酸化蛋白质组在香蕉。以及它们对冷应激的调节作用。本研究对冷敏感型卡文迪什香蕉和相对耐冷的大蕉在低温胁迫下的磷酸化蛋白质组进行了比较分析。MKK2相互作用网络中5种磷蛋白（MKK2、HY5、CaSR、STN7和类驱动蛋白）的磷肽丰度在卡文迪什香蕉和大蕉中存在显著差异。MKK 2蛋白及其T31磷酸化肽的Western印迹证实了在一段时间的冷胁迫过程中，道滘中T31磷酸肽丰度增加而MKK 2丰度降低的磷酸化蛋白质组学结果。同时，在卡文迪什香蕉中发现MKK 2的表达增加，而没有检测到T31磷酸化。这些结果表明，MKK 2途径，沿着其他冷特异性磷蛋白，似乎与大蕉高耐冷胁迫的分子机制。这些结果也提供了新的证据，细胞MKK 2磷酸化的信号通路在非生物胁迫耐受性中起着重要作用，可能是一种普遍的植物耐冷机制。
Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes inMusa spp. and their regulatory roles in response to cold stress. In this study, we conducted a comparative phosphoproteomic profiling of cold-sensitive Cavendish Banana and relatively cold tolerant Dajiao under cold stress. Phosphopeptide abundances of five phosphoproteins involved in MKK2 interaction network, including MKK2, HY5, CaSR, STN7 and kinesin-like protein, show a remarkable difference between Cavendish Banana and Dajiao in response to cold stress. Western blotting of MKK2 protein and its T31 phosphorylated peptide verified the phosphoproteomic results of increased T31 phosphopeptide abundance with decreased MKK2 abundance in Daojiao for a time course of cold stress. Meanwhile increased expression of MKK2 with no detectable T31 phosphorylation was found in Cavendish Banana. These results suggest that the MKK2 pathway in Dajiao, along with other cold-specific phosphoproteins, appears to be associated with the molecular mechanisms of high tolerance to cold stress in Dajiao. The results also provide new evidence that the signaling pathway of cellular MKK2 phosphorylation plays an important role in abiotic stress tolerance that likely serves as a universal plant cold tolerance mechanism.