Preconception zinc deficiency disrupts postimplantation fetal and placental development in mice.
Preconception zinc deficiency disrupts postimplantation fetal and placental development in mice.
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孕前缺锌会扰乱小鼠植入后胎儿和胎盘的发育。
DOI：
10.1095/biolreprod.113.113910
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发表时间：
2014
影响因子：
3.6
通讯作者：
Diaz,FranciscoJ
中科院分区：
文献类型：
--
作者：
Tian,Xi;Anthony,Kate;Neuberger,Thomas;Diaz,FranciscoJ
锌是最佳生育能力的必需营养素，但孕前缺锌对着床后发育的影响尚不清楚。雌性小鼠在排卵前（受孕前）喂食对照或缺锌饮食 (ZDD) 4-5 天。在妊娠第 3.5、6.5、10.5、12.5 和 16.5 天评估胚胎和/或胎盘发育。研究结果显示，接受 ZDD 孕前喂养的母亲的胚胎长度（第 10.5 天，减少 31%；第 12.5 天，减少 13%；第 16.5 天，减少 10%）和重量（第 16.5 天，减少 23%）。与对照组（第 10.5 天为 2%；第 12.5 天为 7%；第 16.5 天为 9%）相比，缺锌也导致流产率较高（第 10.5 天为 46%；第 12.5 天为 34%；第 16.5 天为 51%）。与对照组的 40% 相比，移植到正常受体的 ZDD 胚胎小了 38%，植入率仅为 10%。妊娠第 6.5 天的滋养层细胞分化和植入因孕前缺锌而受到影响。怀孕第12.5天，由于孕前缺锌，胎盘重量和胎儿胎盘面积分别减少了37%和31%。与较小的胎儿胎盘一致，ZDD 组中关键胎盘转录物的表达，包括 Ar、Esx1、Syna、Tfeb、Dlx3 和 Gcm1 mRNA，但不包括 CtsqmRNA，降低了 30%–70%。通过光学显微镜和磁共振成像检查，孕前缺锌导致 41%–57% 的胚胎表现出神经管发育延迟或异常。总的来说，这些发现为孕前锌在促进最佳生育能力和卵母细胞发育潜力方面的重要性提供了证据。
Zinc is an essential nutrient for optimal fertility, but the effects of preconception zinc deficiency on postimplantation development are not known. Female mice were fed a control or a zinc-deficient diet (ZDD) for 4–5 days before ovulation (preconception). Embryonic and/or placental development were evaluated on Days 3.5, 6.5, 10.5, 12.5, and 16.5 of pregnancy. The findings show a decrease in embryo length (31%, Day 10.5; 13%, Day 12.5; 10%, Day 16.5) and weight (23%, Day 16.5) in embryos from mothers fed a ZDD preconception. Zinc deficiency also caused a high incidence of pregnancy loss (46%, Day 10.5; 34%, Day 12.5; 51%, Day 16.5) compared to control (2%, Day 10.5; 7%, Day 12.5; 9%, Day 16.5). ZDD embryos transferred to normal recipients were 38% smaller and implantation rate was only 10% compared to 40% for controls. Trophoblast cell differentiation and implantation on Day 6.5 of pregnancy were compromised by preconception zinc deficiency. On Day 12.5 of pregnancy, placenta weight and area of fetal placenta were decreased 37% and 31%, respectively, by preconception zinc deficiency. Consistent with a smaller fetal placenta, expression of key placental transcripts, includingAr,Esx1,Syna,Tfeb,Dlx3, andGcm1mRNA, but notCtsqmRNA, were decreased 30%–70% in the ZDD group. Preconception zinc deficiency caused 41%–57% of embryos to exhibit delayed or aberrant neural tube development, as examined by light microscopy and magnetic resonance imaging. Collectively, the findings provide evidence for the importance of preconception zinc in promoting optimal fertility and oocyte developmental potential.