Targeted Photoswitchable Probe for Nanoscopy of Biological Structures
Targeted Photoswitchable Probe for Nanoscopy of Biological Structures
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DOI：
10.1002/cbic.201000189
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发表时间：
2010-07-05
期刊：
影响因子：
3.2
通讯作者：
Lasser, Theo
中科院分区：
文献类型：
--
作者：
Dellagiacoma, Claudio;Lukinavicius, Grazvydas;Lasser, Theo
SNAP标签：我们介绍了一种可光开关的O 6-苄基鸟嘌呤衍生物，并证明了其用于基于单荧光团定位的SNAP标记蛋白质的超分辨率显微镜。随机光学重建显微镜（STORM）显示SNAP标记的微管结构，分辨率为1.25 nm。所描述的探针与多功能SNAP标签标记相结合，为在纳米级成像生物结构开辟了新的可能性。
SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with∼ 25 nm resolution. The described probe in combination with the versatile SNAP‐tag labeling opens new possibilities for imaging biological structures at the nanoscale.