Identification of low-temperature-regulated ORFs in the cyanobacterium Anabaena sp Strain PCC 7120:: Distinguishing the effects of low temperature from the effects of photosystem II excitation pressure
Identification of low-temperature-regulated ORFs in the cyanobacterium Anabaena sp Strain PCC 7120:: Distinguishing the effects of low temperature from the effects of photosystem II excitation pressure
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DOI：
10.1093/pcp/pci132
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发表时间：
2005-08-01
影响因子：
4.9
通讯作者：
Sato, N
中科院分区：
文献类型：
--
作者：
Ehira, S;Ohmori, M;Sato, N
大多数生物都发展出各种策略来快速应对温度下降，并调节各种基因的表达以适应低温。在光合生物中，光的温度下移不仅会导致生长温度的降低，还会导致PSII激发压力的增加。区分低温效应和激励压力效应是理解低温信号转导机制的必要条件。本研究利用DNA芯片技术，分析了蓝藻Anabaena sp.菌株PCC 7120在光照下温度下降、黑暗下温度下降和向黑暗转移三种不同环境变化后基因表达的变化。通过比较三种实验条件下的表达模式，我们发现15个开放阅读框（orf）在光照和黑暗条件下都受到温度下调的上调。这些orf被认为是由低温调节的，而不是由激发压力调节的。其中6个在其编码区的上游区域有一个一致的序列，并且也被四环素上调。核糖体的功能或结构变化可能影响低温调控orf的转录水平。
Most organisms have developed various strategies to react rapidly to temperature down-shift and regulate expression of various genes to acclimate to low temperature. In photosynthetic organisms, temperature down-shift in the light results in not only a decrease in growth temperature but also an increase in PSII excitation pressure. Distinguishing the effects of low temperature from the effects of excitation pressure is necessary to understand the mechanism of low-temperature signal transduction. In this report, we analyzed changes in gene expression after three different environmental changes, i.e. temperature down-shift in the light, temperature down-shift in the dark and transfer to the dark, using DNA microarray in the cyanobacterium Anabaena sp. strain PCC 7120. By comparing the expression patterns under the three experimental conditions, we identified 15 open reading frames (ORFs) that were up-regulated by temperature down-shift both in the light and in the dark. These ORFs are considered to be regulated by low temperature, but not by excitation pressure. Six of them have a consensus sequence within the upstream region of their coding region and were indicated also to be up-regulated by tetracycline. Functional or structural changes in the ribosome could affect transcript levels of the low-temperature-regulated ORFs.