HLA-DQB1 CAR1/CAR2, TNFa IR2/IR4 and CTLA-4 polymorphisms in Tunisian patients with rheumatoid arthritis and Sjogren's syndrome
HLA-DQB1 CAR1/CAR2, TNFa IR2/IR4 and CTLA-4 polymorphisms in Tunisian patients with rheumatoid arthritis and Sjogren's syndrome
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DOI：
10.1093/rheumatology/40.12.1370
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发表时间：
2001-12-01
期刊：
影响因子：
5.5
通讯作者：
Ayadi, H
中科院分区：
文献类型：
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作者：
Kacem, HH;Kaddour, N;Ayadi, H
目标。目的探讨人类白细胞抗原II类基因和CTLA-4基因在突尼斯人群类风湿性关节炎(RA)和干燥综合征(SS)遗传易感性中的作用。采用特异性聚合酶链式反应(PCR)技术，分析CTLA-4基因外显子3‘-非翻译区的(CA)(N)微卫星、TNFa IR2/IR4和(AT)(N)微卫星的多态性。采用聚合酶链式反应-限制性片段长度多态性方法对CTLA-4A/G外显子1进行基因分型。对60例RA患者、58例SS患者和150例健康人的基因组DNA进行了基因分型。两种疾病患者与对照组之间的HLA-DQ、CAR1/CAR2等位基因频率分布差异均有统计学意义(P
Objectives. To evaluate the contribution of HLA class II region and the CTLA-4 gene in genetic susceptibility to rheumatoid arthritis (RA) and Sjogren's syndrome (SS) in the Tunisian population.Methods. The polymorphisms of a (CA)(n) microsatellite of HLA-DQB1 CAR1/CAR2, TNFa IR2/IR4 and an (AT)(n) microsatellite in the 3'-untranslated region of exon 3 of the CTLA-4 gene were analysed after specific polymerase chain reaction (PCR) amplification. Typing of CTLA-4 A/G exon 1 polymorphism was achieved by the PCR-restriction fragment length polymorphism method.Results. Genomic DNA from 60 patients with RA, 58 patients with SS and 150 healthy individuals was genotyped. The distribution of HLA-DQ CAR1/CAR2 allele frequencies differed between patients and controls in both diseases (RA, P