Response of Candidatus Liberibacter Asiaticus-Infected Citrus Plants to Bacillus amyloliquefaciens GJ1
Response of Candidatus Liberibacter Asiaticus-Infected Citrus Plants to Bacillus amyloliquefaciens GJ1
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受亚洲自由杆菌感染的柑橘植物对解淀粉芽孢杆菌 GJ1 的反应
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发表时间：
2018-03
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通讯作者：
JIANG L
中科院分区：
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作者：
Tang JZ;Ding YX;Deng L;Nan J;Yang XY;Zhao XY;JIANG L
黄龙病是柑橘生产上的一种主要病害。本研究以一株具有抗HLB潜力的新型生防菌解淀粉芽孢杆菌GJ 1为材料进行了抗病性试验。利用等量异序标签进行相对和绝对定量，比较分析了B处理和未处理的黄龙病叶片的蛋白质丰度。解淀粉菌GJ 1（OD 600 nm）。结果显示，在21个表达上调（FC>1.2，P值<0.05）和43个表达下调（FC≤0.83，P值<0.05）的菌株中，获得了差异表达蛋白（DAP）和COG分类注释蛋白。其中，分析了参与碳水化合物运输和代谢的蛋白质。解淀粉芽孢杆菌GJ 1处理导致叶片中淀粉和可溶性糖含量的降低，证明了B对淀粉的降解作用。根中的I2-KI试验证明了解淀粉菌GJ 1处理。本研究突出了柑橘HLB生物防治的原因之一
Huanglongbing (HLB) is a major disease limiting citrus production worldwide. In this study, a new potential biocontrol agent Bacillus amyloliquefaciens GJ1 against HLB was employed to resistance disease experiment. We utilized isobaric tags for relative and absolute quantification-based quantitative proteomics approach for comparative analysis of protein abundances with Candidatus Liberibacter asiaticus-infected leaves treated and untreated by solution of B. amyloliquefaciens GJ1 (OD600 nm ≈ 1). The result revealed Differential Abundance Protein (DAP) of 21 expression-increased (FC>1.2, P-value<0.05) and 43 expression-decreased (FC≤0.83, P-value<0.05) and COG classification annotation proteins were elucidated. Among them, the proteins involved in carbohydrate transport and metabolism were analyzed. Bacillus amyloliquefaciens GJ1 treatment brought about decreasing both of the content of the starch and the soluble sugar in leaves was proved, the function of starch degradation caused by B. amyloliquefaciens GJ1 treatment was proven via I2-KI test in roots. This study highlight one of the reasons in HLB biocontrol in citrus