Identification and mapping of microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in Acala NemX cotton (Gossypium hirsutum L.)
Identification and mapping of microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in Acala NemX cotton (Gossypium hirsutum L.)
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DOI：
10.1007/s00122-005-0183-0
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发表时间：
2006-02
影响因子：
5.4
通讯作者：
C. Wang;M. Ulloa;P. Roberts
中科院分区：
文献类型：
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作者：
C. Wang;M. Ulloa;P. Roberts
寄主植物抗性是防治棉花根结线虫病最经济有效的策略。与抗性连锁的分子标记对于将抗性基因整合到优良品种中是重要的。筛选与水稻抗粒系RKN紧密连锁的微卫星标记。希尔苏图姆阿卡拉NemX、F1、F2、BC 1F 1和F2：7个重组自交系（RILs）来自种内杂交，1个F2来自种间杂交。巴巴多斯岛使用Pima S-7。利用群体分离分析法对284个SSR标记进行了筛选，获得了具有丰富信息的SSR标记，这些SSR标记覆盖了棉花所有已知染色体和大部分连锁群。然后将这些SSR标记定位到上述群体上。鉴定出一个共显性SSR标记CIR 316与主效抗性基因（指定为asrken 1）紧密连锁，在阿卡拉NemX和易感的阿卡拉SJ-2中分别产生约221 bp（CIR 316 a）和210 bp（CIR 316 c）的扩增DNA片段。CIR 316 a标记与阿卡拉NemX中抗性基因的连锁距离为2.1-3.3 cM。另外，包括具有松散连锁torkn 1的BNL 1231（图距25.1-27.4 cM）、BNL 1066和CIR 003在内的标记使therkn 1基因定位于棉花连锁群A03。这是第一个报告在棉花与一个紧密连锁的主基因位点决定线虫的抗性，和信息SSR可用于标记辅助选择。
Host-plant resistance is the most economic and effective strategy for root-knot nematode (RKN)Meloidogyne incognitacontrol in cotton (Gossypium hirsutumL.). Molecular markers linked to resistance are important for incorporating resistance genes into elite cultivars. To screen for microsatellite markers (SSR) closely linked to RKN resistance inG. hirsutumcv. Acala NemX, F1, F2, BC1F1, and F2:7recombinant inbred lines (RILs) from intraspecific crosses and an F2from an interspecific cross withG. barbadensecv. Pima S-7 were used. Screening of 284 SSR markers, which cover all the known identified chromosomes and most linkage groups of cotton, was performed by bulked segregant analysis, revealing informative SSRs. The informative SSRs were then mapped on the above populations. One co-dominant SSR marker CIR316 was identified tightly linked to a major resistance gene (designated asrkn1), producing amplified DNA fragments of approximately 221 bp (CIR316a) and 210 bp (CIR316c) in Acala NemX and susceptible Acala SJ-2, respectively. The linkage between CIR316a marker and resistance generkn1in Acala NemX had an estimated distance of 2.1–3.3 cM depending on the population used. Additional markers, including BNL1231 with loose linkage torkn1(map distance 25.1–27.4 cM), BNL1066, and CIR003 allowed therkn1gene to be mapped to cotton linkage group A03. This is the first report in cotton with a closely linked major gene locus determining nematode resistance, and informative SSRs may be used for marker-assisted selection.