In vitro study of gossypol's ovarian toxicity to rodents and goats
In vitro study of gossypol's ovarian toxicity to rodents and goats
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DOI：
10.1016/j.toxicon.2018.02.051
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发表时间：
2018-04-01
期刊：
影响因子：
2.8
通讯作者：
Soto-Blanco, Benito
中科院分区：
文献类型：
--
作者：
Luz, Valesca B.;Gadelha, Ivana Cristina N.;Soto-Blanco, Benito
棉酚干扰生殖，对精子造成损害，扰乱发情周期，导致胚胎死亡。在女性中，棉酚可促进卵巢卵泡变性，但这种作用是直接的还是间接的尚不清楚。因此，本研究的目的是确定棉酚是否干扰体外大鼠、小鼠和山羊的卵泡发生。大鼠、小鼠卵巢和山羊卵巢碎片分别在细胞培养中培养24 h和7 d。4组分别为0（对照）、5、10、20 μ g棉酚/ml。孵育后，将卵巢固定并进行组织学分析。卵泡根据其发育阶段分为活卵泡和闭锁卵泡。研究发现，在大鼠、小鼠和山羊的卵巢中，棉酚在卵泡发育的各个阶段都显示出闭锁卵泡比例的增加和活卵泡比例的降低。与对照组相比，培养24 h后，大鼠、小鼠和山羊各组卵巢卵泡活力分别降低56.9%、56.5%和68.0%，其中棉酚浓度最高（20 μ g/mL），培养7 d后分别降低65.4%、65.3%和88.2%。因此，棉酚可能直接影响卵泡成熟，从而影响女性的生育能力。(C) 2018 Elsevier Ltd.版权所有。
Gossypol interferes with reproduction, causing damage to sperm, disrupting the estrous cycle and resulting in embryonic lethality. In females, gossypol administration promotes degeneration of ovarian follicles, but it is unknown whether this effect is direct or indirect. Thus, the aim of this study is to determine whether gossypol interferes with folliculogenesis in vitro in rats, mice and goats. Ovaries from rats and mice and fragments of goat ovaries were grown in cell culture for 24 h or 7 days. Four groups were tested: 0 (control), 5, 10 and 20 mu g gossypol/ml. After incubation, the ovaries were fixed and processed for histological analysis. Follicles were classified according to their stage of development as either viable or atretic. It was found that the ovaries of rats, mice and goats cultured with gossypol showed an increase in the proportion of atretic follicles and a consequent reduction in the proportion of viable follicles at all stages of follicular development. Compared to the control group, the viability of all ovarian follicles in the rat, mouse and goat groups was reduced after cultivation for 24 h by 56.9%, 56.5% and 68.0%, respectively, with the highest concentration of gossypol (20 mu g/mL), and after seven days, the respective reductions were 65.4%, 65.3% and 88.2%. Thus, it is possible that gossypol may directly affect follicular maturation, and consequently female fertility. (C) 2018 Elsevier Ltd. All rights reserved.