Amylin reduces food intake more potently than calcitonin gene-related peptide (CGRP) when injected into the lateral brain ventricle in rats
Amylin reduces food intake more potently than calcitonin gene-related peptide (CGRP) when injected into the lateral brain ventricle in rats
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DOI：
10.1016/s0196-9781(98)00114-4
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发表时间：
1998-01-01
期刊：
影响因子：
3
通讯作者：
Scharrer, E
中科院分区：
文献类型：
--
作者：
Lutz, TA;Rossi, R;Scharrer, E
胰淀素和结构和功能相关肽降钙素基因相关肽（CGRP）已被证明可以减少大鼠的食物摄入量。本研究的目的是比较两种肽在宽剂量范围内给药至侧脑室（ICV）时的厌食效力。此外，我们还测试了在ICV给药后，孤束最后区/核（AP/NTS）区域中的病变对胰淀素和CGRP的厌食作用的影响，因为AP/NTS病变已显示在腹膜内（IP）注射时降低两种肽的厌食作用。胰淀素[1-510 pmol/大鼠（0.004-2 μ g/大鼠）ICV]和CGRP [1-131 pmol/大鼠（0.004-0.5 μ g/大鼠）ICV]剂量依赖性地减少食物剥夺大鼠的食物摄入。在26 pmol/大鼠（0.1 μ g/大鼠）的剂量下，胰淀素在注射后1小时内几乎完全抑制摄食。胰淀素[EC 50 = 2 pmol/rat（0.007 μ g/rat）]的厌食作用明显强于CGRP [57 pmol/rat（0.215 μ g/rat）]。AP/NTS区域的病变不影响胰淀素和CGRP在侧脑室给药后的厌食作用。可以得出结论，胰淀素比CGRP更有效地减少食物摄入后，管理到侧脑室。前脑中的受体可以介导通过该途径给药时两种肽的厌食作用。(C)1998年爱思唯尔科学公司
Amylin and the structurally and functionally related peptide calcitonin gene-related peptide (CGRP) have been shown to reduce food intake in rats. The aim of the present study was to compare the anorectic potency of both peptides over a wide dose range when administered into the lateral brain ventricle (ICV). Furthermore, we also tested the influence of a lesion in the area postrema/nucleus of the solitary tract (AP/NTS) region on the anorectic effects of amylin and CGRP after ICV administration because AP/NTS lesion has been shown to reduce the anorectic effects of both peptides when injected intraperitoneally (IP). Amylin [1-510 pmol/rat (0.004-2 mu g/rat) ICV] and CGRP [1-131 pmol/rat (0.004-0.5 mu g/rat) ICV] dose-dependently reduced food intake in food-deprived rats. At a dose of 26 pmol/rat (0.1 mu g/rat), amylin almost completely suppressed food intake for 1 h after injection. Amylin [EC50 = 2 pmol/rat (0.007 mu g/rat)] was markedly more potent than CGRP [57 pmol/rat (0.215 mu g/rat)] with regard to its anorectic effect. A lesion in the AP/NTS region did not influence the anorectic effects of amylin and CGRP after administration into the lateral ventricle. It is concluded that amylin is more potent than CGRP in reducing food intake after administration into the lateral brain ventricle. Receptors in the forebrain may mediate the anorectic effects of both peptides when administered via this route. (C) 1998 Elsevier Science Inc.