Mitogenic response to epidermal growth factor (EGF) modulated by platelet-derived growth factor in cultured fibroblasts.
Mitogenic response to epidermal growth factor (EGF) modulated by platelet-derived growth factor in cultured fibroblasts.
复制标题
在培养的成纤维细胞中，血小板衍生生长因子对表皮生长因子（EGF）的有丝分裂反应。
DOI：
10.1016/0014-4827(83)90226-4
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发表时间：
1983
影响因子：
3.7
通讯作者：
Pledger,WJ
中科院分区：
文献类型：
--
作者：
Wharton,W;Leof,E;Olashaw,N;O'Keefe,EJ;Pledger,WJ
本文观察了表皮生长因子（EGF）对BALB/C-3 T3和C3 H10 T1/2两种成纤维细胞系的有丝分裂作用。将EGF（30 ng/ml）加入到含有贫血小板血浆或10− 5 M胰岛素的培养基中的静止3 T3细胞中，仅引起刺激启动DNA合成的细胞百分比的微小增加。与此相反，EGF与胰岛素或血浆协同作用，以刺激DNA合成在静止培养的10 T1/2细胞，虽然EGF的最大影响是在浓度的几倍以上，在血清或血浆中发现的。在3 T3或10 T1/2细胞中，短暂预暴露于血小板衍生生长因子（PDGF）导致对EGF促有丝分裂作用的敏感性增加10倍以上。因此，PDGF的主要作用可能是增加成纤维细胞对EGF的敏感性，而与单独的EGF是否具有促有丝分裂作用无关。
The mitotic effects of epidermal growth factor (EGF) were investigated in two cultured fibroblast lines, BALB/C-3T3 and C3H 10T1/2 cells. EGF (30 ng/ml) added to quiescent 3T3 cells in medium containing either platelet-poor plasma or 10−5M insulin caused only minimal increases in the percentage of cells stimulated to initiate DNA synthesis. In contrast, EGF acted synergistically with either insulin or plasma to stimulate DNA synthesis in quiescent cultures of 10T1/2 cells, although the maximum effects of EGF were measured at concentrations several-fold greater than those found in either serum or plasma. In either 3T3 or 10T1/2 cells a transient preexposure to platelet-derived growth factor (PDGF) caused over a 10-fold increase in the sensitivity to the mitogenic effects of EGF. It is therefore possible that a primary action of PDGF is to increase the sensitivity of fibroblasts to EGF, independent of whether EGF alone is found to be mitogenic.