Sequencing and Phylogenetic Analysis of the Coding Region of Six Common Rotavirus Strains: Evidence for Intragenogroup Reassortment Among Co-Circulating G1P[8] and G2P[4] Strains From the United States
Sequencing and Phylogenetic Analysis of the Coding Region of Six Common Rotavirus Strains: Evidence for Intragenogroup Reassortment Among Co-Circulating G1P[8] and G2P[4] Strains From the United States
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DOI：
10.1002/jmv.21977
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发表时间：
2011-03-01
影响因子：
12.7
通讯作者：
Gentsch, J. R.
中科院分区：
文献类型：
--
作者：
Banyai, K.;Mijatovic-Rustempasic, S.;Gentsch, J. R.
轮状病毒的分段基因组为轮状病毒毒株通过重配产生大的遗传多样性提供了机会;然而，这种机制被认为在除中和抗原以外的基因中在Wa样和DS-1样毒株之间产生嵌合基因星座中起的作用很小。进行了一项初步研究，以在基因组水平上分析这两种流行病学上重要的菌株，以便（i）鉴定基因组间重配和（ii）为未来的基因组学分析提供G1 P [8]和G2 P [4]的额外参考基因组序列。3 G1 P的全部11个基因的完整或几乎完整的编码区[8]（LB 2719、LB 2758和LB 2771）和3 G2 P [4]（LB 2744、LB 2764和LB 2772）菌株，分离自加州长滩因严重腹泻住院的儿童，这些菌株在2005-2006年期间以可比的速率传播的地方在本研究中描述。基于全基因组分类系统，所有G1 P [8]菌株都具有保守的基因组星座：G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-E1-H1，并且与基因组序列已经确定的少数Wa样菌株基本相同。同样，3种G2 P [4]菌株的基因组序列高度保守：G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-E2-H2，与参考DS-1样菌株的遗传差异总体较小。虽然在本研究的G1 P [8]和G2 P（4）菌株之间未观察到基因组间重配，但发现了基因组内重配事件的证据。后轮状病毒基因组时代的类似研究将有助于揭示影响骨架基因的基因组间重配是否可以通过逃避接种儿童的免疫力在任何潜在的疫苗突破事件中发挥重要作用。医学病毒学杂志33：532- 539，2011. (C)2011 Wiley-Liss，Inc.
The segmented genome of rotaviruses provides an opportunity for rotavirus strains to generate a large genetic diversity through reassortment; however, this mechanism is considered to play little role in the generation of mosaic gene constellations between Wa-like and DS-1-like strains in genes other than the neutralization antigens. A pilot study was undertaken to analyze these two epidemiologically important strains at the genomic level in order to (i) identify intergenogroup reassortment and (ii) to make available additional reference genome sequences of G1P[8] and G2P[4] for future genomics analyses. The full or nearly complete coding region of all 11 genes for 3 G1P[8] (LB2719, LB2758, and LB2771) and 3 G2P[4] (LB2744, LB2764, and LB2772) strains isolated from children hospitalized with severe diarrhea in Long Beach, California, where these strains were circulating at comparable rates during 2005-2006 are described in this study. Based on the full-genome classification system, all G1P[8] strains had a conserved genomic constellation: G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-E1-H1 and were mostly identical to the few Wa-like strains whose genome sequences have already been determined. Similarly, the genome sequences of the 3 G2P[4] strains were highly conserved: G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-E2-H2 and displayed an overall lesser genetic divergence with reference DS-1-like strains. While intergenogroup reassortment was not seen between the G1P[8] and G2P(4] strains studied here, evidence for intragenogroup reassortment events was identified. Similar studies in the post-rotavirus genomic era will help uncover whether intergenogroup reassortment affecting the backbone genes could play a significant role in any potential vaccine breakthrough events by evading immunity of vaccinated children. J. Med. Virol. 33:532-539,2011. (C) 2011 Wiley-Liss, Inc.