Hydrogels: A potential platform for induced pluripotent stem cell culture and differentiation
Hydrogels: A potential platform for induced pluripotent stem cell culture and differentiation
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DOI：
10.1016/j.colsurfb.2021.111991
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发表时间：
2021-07-29
影响因子：
5.8
通讯作者：
Mony，Ullas
中科院分区：
文献类型：
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作者：
Poorna，M. R.;Jayakumar，R.;Mony，Ullas
诱导多能干细胞（iPSC）可用于产生属于三个胚层（即，外胚层、内胚层和中胚层）。这些细胞在再生医学中具有巨大的潜力。在将iPSC用于各种生物医学应用之前，必须改进现有的异种培养方法，以满足安全性，成本效益和易于处理的技术标准。除了常用的2D基质外，模拟组织中天然细胞环境的培养系统将是培养iPS细胞并将其分化为不同谱系的良好选择。水凝胶是再现天然复杂三维微环境的潜在候选者。它们具有与许多软组织相似的机械特性。此外，水凝胶支持iPSC粘附、增殖和分化为各种细胞类型。它们不含异种，具有成本效益。除了其他基质，如小鼠胚胎成纤维细胞（MEF）、基质胶和玻连蛋白之外，使用基于水凝胶的基质进行iPSC培养和分化可以帮助产生大量的临床级细胞，这些细胞可以用于潜在的临床应用。本文综述了水凝胶在诱导多能干细胞分化中的应用及其在再生医学中的应用。
Induced pluripotent stem cells (iPSCs) can be used to generate desired types of cells that belong to the three germ layers (i.e., ectoderm, endoderm and mesoderm). These cells possess great potential in regenerative medicine. Before iPSCs are used in various biomedical applications, the existing xenogeneic culture methods must be improved to meet the technical standards of safety, cost effectiveness, and ease of handling. In addition to commonly used 2D substrates, a culture system that mimics the native cellular environment in tissues will be a good choice when culturing iPS cells and differentiating them into different lineages. Hydrogels are potential candidates that recapitulate the native complex three-dimensional microenvironment. They possess mechanical properties similar to those of many soft tissues. Moreover, hydrogels support iPSC adhesion, proliferation and differentiation to various cell types. They are xeno-free and cost-effective. In addition to other substrates, such as mouse embryonic fibroblast (MEF), Matrigel, and vitronectin, the use of hydrogel-based substrates for iPSC culture and differentiation may help generate large numbers of clinical-grade cells that can be used in potential clinical applications. This review mainly focuses on the use of hydrogels for the culture and differentiation of iPSCs into various cell types and their potential applications in regenerative medicine.