Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene
Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene
复制标题
DOI：
10.1007/s10096-007-0254-z
复制
发表时间：
2007-02-01
影响因子：
4.5
通讯作者：
Linde, H.
中科院分区：
文献类型：
--
作者：
Reischl, U.;Tuohy, M. J.;Linde, H.
为了评估靶向Panton-Valentine杀白细胞素（PVL）阳性金黄色葡萄球菌的lukS-PV基因的实时PCR检测的速度和准确性，700 S.检测金黄色葡萄球菌菌株，并将结果与阻断循环PCR获得的结果进行比较。用166种其他细菌物种测试交叉反应性。使用这种同质实时PCR检测格式，存在或不存在的遗传信息PVL，这也发现在社区相关的耐甲氧西林S。金黄色葡萄球菌，从纯培养物中正确鉴定，并直接在各种类型的临床标本。
In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.