MicroRNA profiling of human intrahepatic cholangiocarcinoma cell lines reveals biliary epithelial cell-specific microRNAs.
MicroRNA profiling of human intrahepatic cholangiocarcinoma cell lines reveals biliary epithelial cell-specific microRNAs.
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DOI：
10.1272/jnms.76.188
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发表时间：
2009-08
期刊：
影响因子：
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通讯作者：
Yutaka Kawahigashi;T. Mishima;Y. Mizuguchi;Y. Arima;S. Yokomuro;Tomohiro Kanda;O. Ishibashi;H. Yoshida;T. Tajiri;T. Takizawa
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文献类型：
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作者：
Yutaka Kawahigashi;T. Mishima;Y. Mizuguchi;Y. Arima;S. Yokomuro;Tomohiro Kanda;O. Ishibashi;H. Yoshida;T. Tajiri;T. Takizawa
肝内胆管细胞癌（ICC），发生于肝脏的小胆管，是第二常见的肝脏恶性肿瘤。尽管microRNA（miRNA）表达的调节已被证明是恶性肿瘤的有力标志，但ICC的miRNA谱仍不清楚。我们对2个ICC细胞系（HuCCT 1和MEC）和1个正常肝内胆管上皮细胞系（HIBEpiC）的小RNA文库进行测序分析，以产生体外ICC的miRNA谱。此外，通过实时聚合酶链反应（PCR），我们验证了差异表达的miRNAs克隆专门或主要从每个细胞系。从这3种细胞系中共获得35，759个小RNA克隆。我们鉴定了27种在每个细胞系中专门或主要表达的miRNAs。随后的实时PCR验证证实，miRNAs hsa-miR-22、-125 a、-127、-199 a、-199 a *、-214、-376 a和-424在HIBEpic中特异性表达，但在ICC细胞系中下调。我们的研究为促进研究miRNAs在肝胆系统癌变中的功能作用提供了重要信息。本研究中发现的胆管上皮细胞特异性miRNAs可能作为ICC的潜在生物标志物。
Intrahepatic cholangiocarcinoma (ICC), which arises in the small bile ducts of the liver, is the second most common liver malignancy. Although modulation of microRNA (miRNA) expression has been shown to be a potent sign of malignant tumors, miRNA profiles of ICC remains unclear. We performed sequencing analysis of the small RNA libraries of 2 ICC cell lines (HuCCT1 and MEC) and one normal intrahepatic biliary epithelial cell line (HIBEpiC) to produce the miRNA profiles of ICC in vitro. Furthermore, by means of the real-time polymerase chain reaction (PCR) we validated the differential expression of miRNAs cloned exclusively or predominantly from each of the cell lines. A total of 35,759 small RNA clones were obtained from the 3 cell lines. We identified 27 miRNAs that were expressed exclusively or predominantly in each cell line. Subsequent validation with the real-time PCR confirmed that the miRNAs hsa-miR-22, -125a, -127, -199a, -199a*, -214, -376a, and -424 were expressed specifically in HIBEpiC but were downregulated in the ICC cell lines. Our study provides important information for facilitating studies of the functional role(s) of miRNAs in carcinogenesis of the hepatobiliary system. The biliary epithelial cell-specific miRNAs identified in this study may serve as potential biomarkers for ICC.