An efficient procedure for cloning hormone-responsive genes from a specific tissue.
An efficient procedure for cloning hormone-responsive genes from a specific tissue.
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从特定组织克隆激素反应基因的有效程序。
DOI：
10.1089/10445490050128421
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Saatcioglu,F
中科院分区：
文献类型：
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作者：
Korkmaz,KS;Korkmaz,CG;Ragnhildstveit,E;Pretlow,TG;Saatcioglu,F
核受体形成配体激活转录因子的超家族。与近年来在剖析核受体结构及其相应功能方面取得的重大进展相比，核受体靶基因的鉴定进展相当缓慢，而核受体的靶基因是理解激素作用的先决条件。在这里，我们描述了一个简单的筛选方案，可以有效地克隆特定于感兴趣组织的激素反应基因。当该程序用于克隆前列腺特异性和雄激素反应基因时，随机选择的约 40% 的克隆代表已知受雄激素调节且很大程度上对前列腺表达具有特异性的基因，例如前列腺特异性抗原 (PSA)。另外 37% 已知在前列腺中高度富集表达，但它们的雄激素调节仍有待研究。其余克隆代表新基因、表达序列标签或尚未评估其可能的雄激素调节作用的已知基因。该筛选方案可应用于任何激素/配体，以克隆特定于感兴趣组织的差异表达基因。这些基因的鉴定及其特征应该极大地有助于理解正常和病理条件下的激素作用。
Nuclear receptors form a superfamily of ligand-activated transcription factors. In contrast to the significant advances made in recent years to dissect nuclear receptor structure and their corresponding function, progress has been rather slow in the identification of target genes for nuclear receptors, information that is a prerequisite for understanding hormone action. Here, we describe a simple screening protocol that makes it possible to efficiently and effectively clone hormone-responsive genes that are specific to a tissue of interest. When this procedure was used to clone prostate-specific and androgen-responsive genes, approximately 40% of the clones selected at random represented genes that are known to be androgen regulated and are largely specific to prostate for expression, such as prostate specific antigen (PSA). A further 37% are known to be highly enriched in prostate for expression, but their androgen regulation is yet to be studied. The rest of the clones represented novel genes, expressed sequence tags, or known genes whose possible androgen regulation has not yet been assessed. This screening scheme can be applied to any hormone/ligand to clone differentially expressed genes specific to a tissue of interest. Identification of such genes and their characterization should greatly facilitate understanding hormone action in normal and pathological conditions.