Some properties of RNA from Fiji disease subviral particles.
Some properties of RNA from Fiji disease subviral particles.
复制标题
斐济病亚病毒颗粒 RNA 的一些特性。
DOI：
10.1016/0042-6822(75)90124-5
复制
发表时间：
1975
期刊：
影响因子：
3.7
通讯作者：
R. Francki
中科院分区：
文献类型：
--
作者：
M. Ikegami;R. Francki
从斐济病病毒（FDV）亚病毒颗粒中分离的核酸经以下性质鉴定为双链rna:(1) orcinol反应阳性；(2) 1 × SSC（氯化钠-柠檬酸钠缓冲液）对核糖核酸酶（RNase）有抗性，而0.1 × SSC对RNase无抗性；(3) 1 × SSC热变性后对RNase的敏感性；(4)在0.01 × SSC条件下，熔点为76°的尖锐热变性曲线；(5)浮力密度为1.60 g/cm3 Cs2SO4；(6) 37°甲醛处理对紫外线吸收无增加作用。经聚丙烯酰胺凝胶电泳，FDV-RNA分离成9个RNA片段，总分子量为15.3 × 106。
Nucleic acid isolated from subviral particles of Fiji disease virus (FDV) was identified as double-stranded (ds)-RNA by the following properties: (1) Positive orcinol reaction; (2) resistance to ribonuclease (RNase) in 1 × SSC (sodium chloride-sodium citrate buffer) but not in 0.1 × SSC; (3) susceptibility to RNase in 1 × SSC after thermal denaturation; (4) sharp thermal denaturation curve with a melting temperature of 76° in 0.01 × SSC; (5) buoyant density of 1.60 g/cm3in Cs2SO4; and (6) no increase in ultraviolet absorption on treatment with formaldehyde at 37°. On electrophoresis in polyacrylamide gel, FDV-RNA separated into nine RNA segments with a total molecular weight of 15.3 × 106.