Hemoglobin-incorporated iron quantum clusters as a novel fluorometric and colorimetric probe for sensing and cellular imaging of Zn(II) and cysteine
Hemoglobin-incorporated iron quantum clusters as a novel fluorometric and colorimetric probe for sensing and cellular imaging of Zn(II) and cysteine
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DOI：
10.1007/s00604-017-2600-x
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发表时间：
2017
影响因子：
5.7
通讯作者：
Naimeh Hashemi;Z. Vaezi;Mosslim Sedghi;H. Naderi-manesh
中科院分区：
文献类型：
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作者：
Naimeh Hashemi;Z. Vaezi;Mosslim Sedghi;H. Naderi-manesh
作者描述了一种新型的水溶性、稳定性、生物相容性和高荧光的人成人血红蛋白(HB-FeQCs)探针(HB-FeQCs)。用多种光谱技术对HB-FeQC进行了表征。该探针具有较强的吸收和黄色荧光，中心波长为567 nm(光激发波长为460 nm)。HB-FeQCs在很宽的pH值范围内(5-12)表现出优异的光稳定性，甚至在高电解液浓度下也是如此。建立了水溶液中锌(II)和半胱氨酸的比色法和荧光法。锌离子会导致可见的颜色从棕色变为黄色。除Co2+和Cu2+对其他金属离子有轻微干扰外，HB-FeQCs对其他金属离子的灵敏度可忽略不计。利用HB-FeQCs对锌离子进行了灵敏和选择性的开启荧光分析。半胱氨酸对HB-FeQCs/Zn(II)络合物的荧光有猝灭作用。在优化的条件下，该探针在0.04~2.2Mμ的锌(II)浓度范围内具有良好的线性响应，检出限为48 nm。半胱氨酸对半胱氨酸的响应在1-60μM浓度范围内呈线性关系，检出限为0.25μM。该荧光探针在可见光下与活体人成纤维细胞中的锌离子结合后，荧光发射强度增强。这种可溶性铁量子簇具有良好的细胞成像能力和极低的细胞毒性，有望成为一种具有生物材料应用前景的亚纳米平台。图形摘要表征了血红蛋白基质中黄色发射铁量子簇(HB-FeQCs)，并成功应用于锌(II)和半胱氨酸的传感。该探测器充当开关荧光探针，可用于在可见光下对人成纤维细胞中的锌离子进行成像。
The authors describe a novel water-soluble, stable, biocompatible, and highly fluorescent probe consisting of iron quantum clusters incorporated into human adult hemoglobin (Hb-FeQCs). The Hb-FeQCs were characterized by various spectroscopic techniques. The probe displays strong absorption and yellow fluorescence with a peak centered at 567 nm (photo-excited at 460 nm). The Hb-FeQCs show excellent photostability over a wide range of pH values (5–12), even in the presence of high electrolyte concentrations. A colorimetric and a fluorometric method were worked out for the quantitation Zn(II) and cysteine in aqueous solution. Zinc ions induce a visible color change from brown to yellow. The sensitivity of Hb-FeQCs towards other metal ions was negligible, with the exception of Co2+and Cu2+, which caused a modest interference. The Hb-FeQCs were exploited in a sensitive and selective turn-on fluorescence assay for Zn2+. It is also found that cysteine quenches the fluorescence of the Hb-FeQCs/Zn(II) complex. Under the optimized conditions, the probe has a linear response in the 0.04 to 2.2 μM Zn(II) concentration range, with a 48 nM detection limit. Response to cysteine is linear in the 1–60 μM concentration range, with a 0.25 μM limit of detection. This fluorescent probe undergoes fluorescent emission intensity enhancement upon binding to zinc ions in living normal human fibroblast cells under visible lamp. The cellular imaging capability and very low cytotoxicity of this soluble iron quantum clusters can be potentially extended as an exciting sub-nanoplatform with promising biomaterial applications.Graphical abstractSchematic of yellow-emitting iron quantum clusters in hemoglobin matrix (Hb-FeQCs) were characterized and successfully applied for sensing zinc(II) and cysteine. The act as an on-off fluorescent probe and can be applied to image zinc ions in human fibroblast cells under visible light.