Cellular composition of the human diabetic pancreas
Cellular composition of the human diabetic pancreas
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人类糖尿病胰腺的细胞组成
DOI：
10.1007/bf00251826
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发表时间：
1983
期刊：
影响因子：
8.2
通讯作者：
J. Henquin
中科院分区：
文献类型：
--
作者：
Jacques Rahier;R. Goebbels;J. Henquin
胰岛素，胰高血糖素，生长抑素和胰腺多肽细胞的免疫过氧化物酶染色技术和定量的胰腺切片中获得的8个对照组，4个1型（胰岛素依赖型）和8个2型（非胰岛素依赖型）糖尿病患者的形态。研究整个胰腺以考虑不同细胞类型的不均匀分布。根据每种细胞类型的体积密度和胰腺各叶的重量，计算内分泌组织的总质量。对照组平均为1395 mg，1型糖尿病患者为413 mg，2型糖尿病患者为1449 mg。在1型患者中观察到的内分泌组织损失几乎限于胰腺多肽细胞贫乏的叶。在这些患者中，B细胞几乎不存在（每个切片最多7个），但“萎缩的胰岛”仍然含有大量的A、D或胰多肽细胞。A、D和胰多肽细胞的质量以及D与A细胞的比例与对照组无差异。这表明1型糖尿病中B细胞的消失对胰腺的任何其他内分泌细胞没有优先影响。在2型糖尿病中，A细胞的质量增加，而B、D和胰多肽细胞的质量没有改变。A细胞的这种增生导致B细胞与A细胞的比率以及D细胞与A细胞的比率降低。这些改变可能会启发2型糖尿病的病理生理学的某些方面。
SummaryInsulin, glucagon, somatostatin and pancreatic polypeptide cells were stained by immunoperoxidase techniques and quantitated morphometrically in sections of pancreases obtained from eight control subjects, four Type 1 (insulin-dependent) and eight Type 2 (non-insulin-dependent) diabetic patients. The whole pancreas was studied to take into consideration the heterogeneous distribution of the different cell types. From the volume density of each cell type, and the weight of each lobe of the pancreas, the total mass of endocrine tissue was calculated. It averaged 1395 mg in control subjects, 413 mg in Type 1 and 1449 mg in Type 2 diabetic patients. The loss of endocrine tissue observed in the Type 1 patients was almost restricted to the lobe poor in pancreatic polypeptide cells. In these patients, B cells were practically absent (at the most seven per section), but the ‘atrophic islets’ still contained numerous A, D, or pancreatic polypeptide cells. The mass of A, D and pancreatic polypeptide cells and the ratio of D to A cells were not different from those measured in the control subjects. This shows that the disappearance of B cells in Type 1 diabetes has no preferential effect on any other endocrine cell of the pancreas. In Type 2 diabetes, the mass of A cells was increased, whereas that of B, D and pancreatic polypeptide cells was not changed. This hyperplasia of A cells leads to a decrease in the ratio of B to A and of D to A cells. These alterations may enlighten certain aspects of the physiopathology of Type 2 diabetes.