Microvessels promote motor nerve survival and regeneration through local VEGF release following ectopic reattachment
Microvessels promote motor nerve survival and regeneration through local VEGF release following ectopic reattachment
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DOI：
10.1080/10739680490517659
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发表时间：
2004-12-01
期刊：
影响因子：
2.4
通讯作者：
Segal, SS
中科院分区：
文献类型：
--
作者：
Bearden, SE;Segal, SS
目的：神经和血管在胚胎发育过程中形成密切联系。生长和引导因素最初被认为是神经或血管的因素，现在已知影响这两个组织，其中最突出的是血管内皮生长因子(VEGF)。作者研究了轴突是否与再生系统中的血管联系，并质疑血管内皮生长因子是否参与了轴突切断后的神经化。方法：在8-12周龄的金黄地鼠上，切断面颊囊牵拉肌的副神经(CN XI)，并将近端残端异位连接到肌肉上。血管铸型和免疫标记用于量化再生过程中神经血管联系的程度。通过对血管内皮生长因子及其受体(SVEGFR2)的免疫定位和对血管内皮生长因子与可溶性受体(SVEGFR1)的局部隔离，研究了血管内皮生长因子在其中的作用。结果：再生轴突与血管神经微血管沿“伴侣”区排列，从再附着点开始生长并进入肌肉。在这个类似于1 mm的区域，生长中的微血管表达了VEGF蛋白，尽管表达减少了50%(p
Objectives: Nerves and blood vessels form a close association during embryogenesis. Growth and guidance factors initially attributed to either nerves or vessels are now known to affect both tissues prominent among these is vascular endothelial growth factor (VEGF). The authors investigated whether axons would associate with blood vessels in a regenerating system and questioned whether VEGF is integral to neurotization following axotomy.Methods: In hamsters 8-12 weeks of age, the accessory nerve (CN XI) to the retractor muscle of the cheek pouch was cut and the proximal stump was reattached ectopically onto the muscle. Vascular casting and immunolabeling were used to quantify, the extent of neurovascular association during regeneration. A role for VEGF was investigated using immunolocalization of VEGF and its receptor (sVEGFR2) as well as local sequestration of VEGF with soluble receptor (sVEGFR1).Results: Regenerating axons aligned with microvessels of the vasa nervorum along a "chaperone" region during outgrowth from the reattachment site and while entering the muscle. In this similar to1-mm region, VEGF protein was expressed by growing microvessels despite a similar to50% reduction (p