ROLE OF IRON AND MOLECULAR OXYGEN IN PULCHERRIMIN SYNTHESIS BY BACTERIA
ROLE OF IRON AND MOLECULAR OXYGEN IN PULCHERRIMIN SYNTHESIS BY BACTERIA
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DOI：
10.1007/bf00406050
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发表时间：
1967-01-01
期刊：
影响因子：
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通讯作者：
CANALEPA.E
中科院分区：
文献类型：
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作者：
KUPFER, DG;UFFEN, RL;CANALEPA.E
蜡状芽孢杆菌和B.通过选择性程序分离枯草芽孢杆菌。对色素进行了纯化和鉴定，确定为Pulcherrimin。铁含量（12.8%）反映了色素的螯合程度，与假丝酵母生产的pulcherrima的螯合程度相同。B菌株不合成Pulcherrimin。cereus和B.在含Fe的缓冲培养基中厌氧生长的枯草芽孢杆菌（培养基中有或没有硝酸盐）。在空气中孵育后，厌氧生长的培养物产生色素。添加抑菌量的氯霉素到厌氧生长的培养物中，防止随后在空气中孵育时色素形成。缺乏O2可能会阻止与Pulcherrimin生产相关的一种或多种酶的合成。在合成在Pulcherrimin生产中起作用的酶之后，生长的细胞可能继续需要O2来形成色素。细胞需氧生长在化学成分确定的培养基中含有微量的铁分泌普氏石蒜氨酸。这些培养物的上清液中加入铁盐导致瞬时Pulcherrimin形成，其不被蛋白质变性剂抑制。Pulcherrimin可能是通过分泌的Pulcherriminic酸和Fe之间的非酶反应在细胞外形成的。铁在pulcherriminic酸的合成中不起调节因子的作用，并且从这种酸形成pulcherrimin对细胞代谢没有显著影响。
Pigment producing strains of Bacillus cereus and B. subtilis were isolated by selective procedures. The pigment was purified and identified as pulcherrimin. The degree of chelation of the pigment, as reflected by the Fe content (12.8%), was identical with that of pulcherrimin produced by Candida pulcherrima. Pulcherrimin was not synthesized by strains of B. cereus and B. subtilis grown anaerobically (with or without nitrate in the medium) in Fe-containing buffered media. Upon incubation in air, anaerobically grown cultures produced pigment. The addition of a bacteriostatic amount of chloramphenicol to anaerobically grown cultures prevented pigment formation upon subsequent incubation in air. The absence of O2 may prevent the synthesis of an enzyme or enzymes associated with pulcherrimin production. After synthesis of the enzymes functioning in pulcherrimin production, growing cells may continue to require O2 for pigment formation. Cells grown aerobically in a chemically defined medium containing trace amounts of Fe excreted pulcherriminic acid. Addition of Fe salts to the supernatant liquid of these cultures resulted in instantaneous pulcherrimin formation which was not inhibited by protein denaturing agents. Pulcherrimin is probably formed extracellularly by a non-enzymatic reaction between excreted pulcherriminic acid and Fe. Iron does not serve as a regulatory factor in the synthesis of pulcherriminic acid, and the formation of pulcherrimin from this acid has no marked effect on cell metabolism.