The water permeability of primary mouse keratinocyte cultures grown at the air-liquid interface.
The water permeability of primary mouse keratinocyte cultures grown at the air-liquid interface.
复制标题
在气液界面生长的原代小鼠角质形成细胞培养物的水渗透性。
DOI：
10.1111/1523-1747.ep12712043
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Potts,RO
中科院分区：
文献类型：
--
作者：
Cumpstone,MB;Kennedy,AH;Harmon,CS;Potts,RO
为了研究体外培养的表皮通透性屏障的发育，在气液界面培养的小鼠角质细胞中测量了氚化水（HTO）通量。使用微扩散技术，结果表明，空气-液体培养形成的水扩散区域与完整的新生小鼠皮肤相当。然而，当在大面积的培养表面上测量水渗透性时，得到的通量明显更高。这些结果表明，在所使用的培养条件下，与完整的新生小鼠皮肤相当的水屏障区域与屏障形成不完全的区域共存。
In order to study the development of the epidermal permeability barrier in vitro, tritiated water (HTO) flux was measured across murine keratinocyte cultured at the air-liquid interface. Using a micro-diffusion technique, it was shown that air-liquid cultures form areas where the water diffusion is comparable to that of intact neonatal mouse skin. When water permeability is measured over a large area of the culture surface, however, significantly higher flux is obtained. These results show that under the culture conditions used, areas of water barrier comparable to intact neonatal mouse skin co-exist with regions of less complete barrier formation.