Differential expression of interleukins (IL) IL-13 and IL-15 throughout the menstrual cycle in endometrium of normal fertile women and women with recurrent spontaneous abortion
Differential expression of interleukins (IL) IL-13 and IL-15 throughout the menstrual cycle in endometrium of normal fertile women and women with recurrent spontaneous abortion
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DOI：
10.1016/s0165-0378(02)00043-8
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发表时间：
2002-07-01
影响因子：
3.4
通讯作者：
Ripps, BA
中科院分区：
文献类型：
--
作者：
Chegini, N;Ma, CF;Ripps, BA
IL-13和IL-15是一种新型的细胞因子和免疫/炎症相关反应的关键调节因子，在各种正常的生物学和相关的子宫内膜异常的转归中起关键作用。本研究通过检测IL-13和IL-15在正常育龄妇女子宫内膜中的表达，探讨其在月经周期中的表达与原因不明反复自然流产(RSA)患者子宫内膜表达的差异。应用定量RT-PCR、ELISA法和免疫组织化学方法，我们发现IL-13和IL-15mRNA和蛋白在整个月经周期和RSA(周期21-23天)中均有表达，表达高峰出现在月经开始后和月经开始前，两个明显的时期分别对应于增殖期晚期和分泌早期-中期。IL-13/IL-15表达的比值显示，IL-13在增殖晚期/分泌早期表达占优势，IL-15在分泌中期表达占优势。与对照子宫内膜相比，RSA患者子宫内膜表达IL-13和IL-15水平升高，IL-13：IL-15比值有利于IL-13。IL-13和IL-15主要定位于子宫内膜腔上皮细胞，在RSA中腺上皮和间质细胞表达增强。综上所述，结果表明正常生育妇女子宫内膜表达IL-13和IL-15，且在月经周期中有不同程度的表达，RSA患者子宫内膜表达升高。尽管IL-13和IL-15在人子宫内膜中的生物学意义以及它们在RSA中的表达上调仍有待研究，但这些具有不同生物学功能的细胞因子可能调节子宫内膜的炎症/免疫反应、组织修复和胚胎着床的接受性。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
Interleukins (IL)-13 and IL-15 are novel cytokines and key regulators of immune/inflammatory related responses that are critical in the outcome of various normal biological and associated abnormalities of the endometrium. The present study determined the temporal and spatial expression of IL-13 and IL-15 mRNA and protein in endometrium of normal fertile women throughout the menstrual cycle, and examined whether profiles of their expression differ from endometrium of women with unexplained recurrent spontaneous abortion (RSA). Using quantitative RT-PCR, ELISA and immunohistochemistry we found that IL-13 and IL-15 mRNA and protein are expressed in control endometrium throughout the menstrual cycle and in RSA (cycle days 21-23) with peak expression detected immediately after and prior to onset of menses, and two distinct periods corresponding to late proliferative and the early-mid secretory phases, respectively. The ratio of IL-13:IL-15 expression revealed a predominance in IL-13 expression during late proliferative/early secretory phase and IL-15 during the mid secretory phase. Compared to control endometrium, endometrium of women with RSA expresses elevated levels of IL-13 and IL-15, with IL-13:IL-15 ratio favoring IL-13. The immunoreactive IL-13 and IL-15 were localized primarily in endometrial luminal epithelial cells with an increased intensity in glandular epithelial and stromal cells in RSA. In conclusion, the results indicate that endometrium of normal fertile women expresses IL-13 and IL-15, with a distinct profile during the menstrual cycle and elevated expression in women with RSA. Although the biological significance of IL-13 and IL-15 in human endometrium and their elevated expression in RSA await investigation, these cytokines with distinct biological functions may regulate endometrial inflammatory/immune responses, tissue repair and receptivity for embryo implantation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.