SCANNING AND TRANSMISSION ELECTRON-MICROSCOPY OF RAT-KIDNEY
SCANNING AND TRANSMISSION ELECTRON-MICROSCOPY OF RAT-KIDNEY
复制标题
DOI：
10.1002/aja.1001390403
复制
发表时间：
1974-01-01
影响因子：
--
通讯作者：
PENDERGRASS, R
中科院分区：
文献类型：
--
作者：
BULGER, RE;SIEGEL, FL;PENDERGRASS, R
本研究利用肾组织的扫描和透射电子显微镜提供了关于肾脏的大体和显微结构的新信息。近曲小管的管腔表面不仅具有微绒毛的边界，而且还具有火山口样凹陷和周向褶皱。在扫描电子显微镜处理的组织中，近端小管细胞沿着其侧面分离，清楚地暴露了细胞质的侧向细胞突起的形貌，由于其与相邻细胞交错，通常无法观察。根据肾单位在肾脏中的位置、一般形态以及顶端微绒毛、纤毛或皮瓣的分布和形式，对肾单位的各个节段进行鉴定。乳头顶端的外表面有几条平行的沟，集合管通向沟内。大斑块样凹陷排列在乳头状表面。肾盂的对侧表面有小斑块样凹陷，由狭窄的脊分隔。先前已通过临界点干燥法脱水的塑料包埋组织样本的透射电子显微镜检查表明，该程序几乎没有发生损伤。
This study utilized scanning and transmission electron microscopy of renal tissue to provide new information on the gross and microscopic structure of the kidney. The luminal surface of the proximal convoluted tubule was characterized not only by the border of microvilli, but also by crater‐like depressions, and circumferential folds. In tissue processed for scanning electron microscopy the proximal tubular cells separated along their lateral surfaces clearly exposing the topography of the lateral cell projections of cytoplasm which have been generally unavailable for viewing because of their interdigitation with adjacent cells. The various segments of the nephron were identified on the basis of position in the kidney, general morphology, and the distribution and form of apical microvilli, cilia, or flaps. The external surface of the papillary tip had several parallel furrows into which the collecting ducts opened. Large plaquelike depressions lined the papillary surface. The opposed surface of the renal pelvis had small plaque‐like depressions separated by narrow ridges. Transmission electron microscopy of plastic‐embedded tissue specimens which had been previously dehydrated by the critical point drying method demonstrated that little damage occurred from this procedure.