Effects of lyophilized black raspberries on azoxymethane-induced colon cancer and 8-hydroxy-2′-deoxyguanosine levels in the Fischer 344 rat
Effects of lyophilized black raspberries on azoxymethane-induced colon cancer and 8-hydroxy-2′-deoxyguanosine levels in the Fischer 344 rat
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DOI：
10.1207/s15327914nc402_8
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发表时间：
2001-01-01
影响因子：
2.9
通讯作者：
Stoner, GD
中科院分区：
文献类型：
--
作者：
Harris, GK;Gupta, A;Stoner, GD
本研究研究了冻干黑树莓（BRB）对偶氮氧甲烷（AOM）诱导的异常隐窝灶（ACF）、结肠肿瘤和雄性Fischer 344大鼠尿8-羟基-2′-脱氧鸟苷（8-OHdG）水平的影响。每周注射1次OM (15 mg/kg体wt / ip)，连续2周。在最后一次注射后24小时，aom处理的大鼠开始食用含有0%、2.5%、5%或10% (wt/wt) BRB的饮食。对照组只接受5% BRB或饮食。BRB喂养9和33周后处死大鼠进行ACF计数和肿瘤分析。2.5%、5%和10% BRB组的ACF多样性分别比纯aom组降低了36%、24%和21% （P < 0.01）。总肿瘤多样性下降42%、45%和71% （P < 0.05）。虽然不显著，但在所有BRB组中均观察到肿瘤负荷降低（28%，42%和75%）。相同治疗组的腺癌多样性分别降低28%、35%和80% （P < 0.01）。尿8-OHdG水平分别降低73%、81%和83%（各组P < 0.01）。这些结果表明，在Fischer 344大鼠中，BRB抑制了aom诱导的几种结肠癌发生，并调节了氧化应激的一个重要标志物。
This study examined the effects of lyophilized black raspberries (BRB) on azoxymethane (AOM)-induced aberrant crypt foci (ACF), colon tumors, and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in male Fischer 344 rats. A OM was injected (15 mg/kg body wt ip) once per week for 2 wk. At 24 h after the final injection, AOM-treated rats began consuming diets containing 0%, 2.5%, 5%, or 10% (wt/wt) BRB. Vehicle controls received 5% BRB or diet only. Rats were sacrificed after 9 and 33 wk of BRB feeding for ACF enumeration and tumor analysis. ACF multiplicity decreased 36%, 24%, and 21% (P < 0.01 for all groups) in the 2.5%, 5%, and 10% BRB groups, respectively, relative to the AOM-only group. Total tumor multiplicity declined 42%, 45%, and 71% (P < 0.05 for all groups). Although-not significant, a decrease in tumor burden (28%, 42%, and 75%) was observed in all BRB groups. Adenocarcinoma multiplicity decreased 28%, 35%, and 80% (P < 0.01) in the same treatment groups. Urinary 8-OHdG levels were reduced by 73%, 81%, and 83% (P < 0.01 for all groups). These results indicate that BRB inhibit several measures of AOM-induced colon carcinogenesis and modulate an important marker of oxidative stress, in the Fischer 344 rat.