Expression of cellular homologues of retroviral onc genes in human hematopoietic cells.
Expression of cellular homologues of retroviral onc genes in human hematopoietic cells.
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逆转录病毒onc基因的细胞同源物在人类造血细胞中的表达。
DOI：
10.1073/pnas.79.8.2490
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发表时间：
1982
影响因子：
11.1
通讯作者：
R. Gallo
中科院分区：
文献类型：
--
作者：
E. Westin;F. Wong;E. Gelmann;R. Dalla;T. Papas;J. Lautenberger;A. Eva;E. Reddy;S. Tronick;S. Aaronson;R. Gallo
分析了来自各种新鲜人白血病血细胞和造血细胞系的总细胞多聚腺苷酸富集RNA与含有Abelson鼠白血病病毒（Ab-MuLV）、哈维鼠肉瘤病毒（Ha-MuSV）、猿猴肉瘤病毒（SSV）和禽骨髓细胞瘤病毒株MC 29的onc序列的分子克隆DNA探针的同源性。新鲜血细胞的结果与细胞系获得的结果一致。使用Ab-MuLV和Ha-MuSV，在检查的所有细胞类型中观察到多条RNA条带，条带的相对强度无显著变化。当SSV被用作探针时，相关onc序列的表达在所有检查的造血细胞类型中均不存在，除了一种肿瘤性T细胞系（HUT 102），其产生人T细胞白血病（淋巴瘤）逆转录病毒HTLV。在该细胞系中，观察到单一条带（4.2个酶）。用MC 29作为探针，在所有检测的细胞类型中观察到2.7个酶的单一条带，杂交强度仅变化10至2倍。一个例外是早幼粒细胞系HL 60，其表达大约10倍以上的MC 29相关onc序列。随着用二甲基亚砜或视黄酸诱导HL 60分化，观察到MC 29相关但非Ab-MuLV相关onc信息的量显著减少。
Total cellular poly(A)-enriched RNA from a variety of fresh human leukemic blood cells and hematopoietic cell lines was analyzed for homology with molecularly cloned DNA probes containing the onc sequence of Abelson murine leukemia virus (Ab-MuLV), Harvey murine sarcoma virus (Ha-MuSV), simian sarcoma virus (SSV), and avian myelocytomatosis virus strain MC29. Results with the fresh blood cells paralleled those obtained with the cell lines. With Ab-MuLV and Ha-MuSV, multiple RNA bands were visualized in all cell types examined without significant variation in the relative intensities of the bands. When SSV was used as the probe, expression of related onc sequences was absent in all of the hematopoietic cell types examined except for one neoplastic T-cell line (HUT 102), which produces the human T-cell leukemia (lymphoma) retrovirus HTLV. In this cell line, a single band (4.2 kilobases) was observed. With MC29 as the probe, a single band of 2.7 kilobases was visualized in all cell types examined with only a 10 to 2-fold variation in intensity of hybridization. An exception was the promyelocytic cell line, HL60, which expressed approximately 10-fold more MC29-related onc sequences. With induction of differentiation of HL60 with either dimethyl sulfoxide or retinoic acid, a marked diminution in the amount of the MC29-related, but not the Ab-MuLV-related, onc message was observed.