Antibody response to GD3 ganglioside is independent of NKT cells.
Antibody response to GD3 ganglioside is independent of NKT cells.
复制标题
对 GD3 神经节苷脂的抗体反应独立于 NKT 细胞。
DOI：
10.1080/14653240701762380
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发表时间：
2008
期刊：
影响因子：
4.5
通讯作者：
Chapman,PB
中科院分区：
文献类型：
--
作者：
Park,J-E;Lu,SX;Wu,DY;Prendes,M;Chapman,PB
nkt细胞识别CD1d在抗原呈递细胞（APC）上呈递的糖脂，并在很大程度上以其被α-半乳糖神经酰胺激活的能力为特征，α-半乳糖神经酰胺是一种在哺乳动物细胞上不表达的糖脂。我们之前已经证明GD3可以通过CD1d交叉呈现给NKT细胞，并且是NKT细胞识别的第一个肿瘤来源的糖脂。但NKT细胞调节b细胞对肿瘤糖脂反应的能力尚未被探索，这些糖脂本身被NKT细胞识别。方法检测GD3抗体（Ab）反应是否需要NKT细胞。我们对野生型小鼠、iNKT细胞缺失小鼠和总NKT细胞缺失小鼠进行GD3免疫。ELISA法检测GD3抗体滴度。结果3株小鼠抗GD3的IgM和IgG反应相似，包括诱导的IgG同种型。对GD3预扩增的NKT细胞不影响抗GD3抗体反应。我们的结论是，抗体对GD3的反应是独立于NKT细胞的，并且在体内操纵NKT细胞的策略不太可能增强疫苗诱导的抗GD3抗体反应。
BackgroundNKT cells recognize glycolipids presented by CD1d on antigen-presenting cells (APC) and have been largely characterized by their ability to be activated by α-galactosylceramide, a glycolipid not expressed on mammalian cells. We have shown previously that GD3 can be cross-presented by CD1d to NKT cells and is the first tumor-derived glycolipid recognized by NKT cells. But the ability of NKT cells to modulate B-cell responses to tumor glycolipids that are themselves recognized by NKT cells has not been explored.MethodsWe tested whether NKT cells are required for antibody (Ab) responses to GD3. We immunized wild-type mice, mice deficient in invariant chain NKT cells (iNKT cells) and mice deficient in total NKT cells against GD3. Ab titer against GD3 was measured by ELISA.ResultsWe found the IgM and IgG responses against GD3 were similar among the three strains of mice, including the IgG isotypes induced. Pre-expanded NKT cells to GD3 did not affect the anti-GD3 Ab response.DiscussionWe conclude that Ab responses to GD3 are independent of NKT cells and that strategies to manipulate NKT cellsin vivoare not likely to enhance the anti-GD3 Ab response induced by vaccines.