Induction of histone acetylation in mouse erythroleukemia cells by some organosulfur compounds including allyl isothiocyanate
Induction of histone acetylation in mouse erythroleukemia cells by some organosulfur compounds including allyl isothiocyanate
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DOI：
10.1002/ijc.1277
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发表时间：
2001-06-15
影响因子：
6.4
通讯作者：
desBordes, C
中科院分区：
文献类型：
--
作者：
Lea, MA;Randolph, VM;desBordes, C
在以前的研究中，我们观察到一些烯丙基硫化物可以引起组蛋白乙酰化增加和DSIP小鼠红白血病细胞分化。在本工作中，我们观察到组蛋白与异硫氰酸烯丙酯和丁硫醇的乙酰化增加，但与丁硫醚和丁二硫醚的乙酰化作用不同。改变凝胶迁移率、掺入[~(3)H]乙酸酯或免疫印迹可以增加组蛋白的乙酰化。0.5 mM的烯丙硫醇和0.5 mM的丁硫醇分别抑制74%和43%的组蛋白去乙酰基酶，但0.5 mM的异硫氰酸烯丙酯对组蛋白脱乙酰基酶的抑制作用不明显。细胞在新鲜培养液中孵育15小时后，异硫氰酸烯丙酯的作用有一定程度的可逆性。提示异硫氰酸烯丙酯可能促进组蛋白乙酰化，而不是抑制组蛋白去乙酰化。然而，添加异硫氰酸烯丙基酯对曲古菌素引起的组蛋白乙酰化的诱导组蛋白乙酰化的影响很小或没有额外的影响。细胞匀浆中组蛋白乙酰转移酶活性的测定不能通过细胞与异硫氰酸烯丙基酯预先孵育或在检测介质中加入异硫氰酸烯丙基酯来增加。结论是，用异硫氰酸烯丙基酯处理小鼠红白血病细胞可以引起组蛋白乙酰化的增加，但这种作用的机制需要进一步的阐明，(C)2001。Inc.
In previous studies we observed that some allyl sulfides can cause increased acetylation of histones and differentiation in DSIP mouse erythroleukemia cells. In the present work we observed increased acetylation of histones with allyl isothiocyanate and butanethiol but not with butyl sulfide or butyl disulfide, Increased acetylation of histones was established by change in electrophoretic mobility, incorporation of [(3)H]acetate or immunoblotting, Histone deacetylase in nuclei of DS19 cells was inhibited 74% by 0.5 mM allyl mercaptan and 43% by 0.5 mM butanethiol but was not significantly affected by 0.5 mM allyl isothiocyanate. There was some degree of reversibility in the effect of allyl isothiocyanate when the cells were incubated for 15 hr in fresh medium. The data suggested that allyl isothiocyanate may stimulate histone acetylation rather than inhibit histone deacetylation. Addition of allyl isothiocyanate, however, had very little or no additional effect on the induction of histone acetylation caused by trichostatin A. Histone acetyltransferase activity determined in cell homogenates was not increased by preincubation of cells with allyl isothiocyanate or inclusion of allyl isothiocyanate in the assay medium, It was concluded that treatment of mouse erythroleukemia cells with allyl isothiocyanate can cause increased acetylation of histones but the mechanism for this effect requires further elucidation, (C) 2001 Wiley-Liss. Inc.