Arabidopsis NDK1 is a component of ROS signaling by interacting with three catalases
Arabidopsis NDK1 is a component of ROS signaling by interacting with three catalases
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DOI：
10.1093/pcp/pcg140
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发表时间：
2003-10-01
影响因子：
4.9
通讯作者：
Hasunuma, K
中科院分区：
文献类型：
--
作者：
Fukamatsu, Y;Yabe, N;Hasunuma, K
植物感知各种环境刺激，并有特定的信号通路对这些信号做出反应。我们专注于光响应成分，并发现NDK在红光照射后在豌豆中被特异性磷酸化[Tanaka等人。(1998)J.PhotoChem。光生物醇。[B 45：113]和蓝光照射后的粗糙脉孢菌[Oda and Hasunuma(1997)Mol.吉内特将军。256：593，Ogura et al.(2001)J.Biol.化学。276：21228]。以拟南芥NDK-P1(豌豆NDK1)的同源基因AtNDK1和分离的Catalase3(AtCat3)为诱饵，进行酵母双杂交筛选。该双杂交系统还检测到AtNDK1-AtCAT1和AtNDK1-AtCAT2之间的相互作用。植物粗提物的非变性双向凝胶电泳法显示，过氧化氢酶和NDK活性在凝胶的同一区域共同迁移。在CaMV 35S启动子控制下表达AtNDK1的转基因植株表现出对百草枯的耐受性和对外源过氧化氢的高清除能力。这些结果表明，AtNDK1在ROS反应中起作用。
Plants sense various environmental stimuli and have specific signaling pathways to respond to these cues. We focused on light responsive components and found that NDKs were phosphorylated specifically after red light irradiation in Pisum sativum [Tanaka et al. (1998) J. Photochem. Photobiol. B 45: 113] and after blue light irradiation in Neurospora crassa [Oda and Hasunuma (1997) Mol. Gen. Genet. 256: 593, Ogura et al. (2001) J. Biol. Chem. 276: 21228]. We performed yeast two-hybrid screening using AtNDK1, the counterpart of NDK-P1 (Pisum sativum NDK1) in Arabidopsis, as bait, and isolated catalase3 (AtCat3). Interactions between AtNDK1-AtCAT1 and AtNDK1-AtCAT2 were also detected with the two-hybrid system. Non-denaturing two-dimensional gel electrophoresis of crude extracts from plants revealed that catalase and NDK activities co-migrated in the same area of the gel. Transgenic plants expressing AtNDK1 under control of the CaMV 35S promoter exhibited tolerance to paraquat and high ability to eliminate exogenous H2O2. These results indicate that AtNDK1 has a role in ROS response.