Sperm DNA damage correlates with polycyclic aromatic hydrocarbons biomarker in coke-oven workers
Sperm DNA damage correlates with polycyclic aromatic hydrocarbons biomarker in coke-oven workers
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DOI：
10.1007/s00420-005-0066-3
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发表时间：
2006-05-01
影响因子：
3
通讯作者：
Wu, MT
中科院分区：
文献类型：
--
作者：
Hsu, PC;Chen, IY;Wu, MT
目的：探讨男性职业性接触多环芳烃(PAHs)是否对精液质量有不良影响。方法：对48名焦炉作业工人进行调查，其中顶炉作业工人16人，侧炉作业工人32人。测定环境中多环芳烃暴露、尿中1-羟基芘(1-OHP)水平和精液质量参数。结果：作业工人暴露于环境中的多环芳烃(3,436.1+/-3,411.0比1,123.1+/-1,829.3 ng/m(3)，P<0.01)，尿中1-羟基苯丙酸水平(207.8+/-176.4比54.0+/-44.8mgg/g，P<；0.001)，少精子发生率(18.8vs.0%，P<；0.05)，精子畸形(32.3vs.14.6%，P<；0.001)；0.01)，而不是工人。在吸烟工人中，TO工人的精子DNA变性率(αT)和DNA变性水平升高的精子百分比(COMPαT)显著高于吸烟工人(αT 246.2+/-49.5vs.198.1+/-30.3COMPαT：34.8%/-14.4vs.19.3+/-13.9%，P<；0.05)。尿1-OHP与αT、COMPαT与精子形态异常呈正相关，尿1-OHP增加10倍，αT增加2.35倍，异常精子百分率增加1.07倍。结论：职业性接触高水平多环芳烃的工人应考虑精子功能障碍的潜在风险。吸烟可能会加剧这种风险。尿1-OHP可作为预测精子功能障碍的生物标志物。
Objectives: The aim was to determine whether occupational exposure to polycyclic aromatic hydrocarbons (PAHs) in men has adverse effect on semen quality. Methods: Forty-eight coke-oven workers, including 16 topside-oven (TO) workers and 32 sideoven (SO) workers, were studied. Ambient PAHs exposure, urinary 1-hydroxypyrene (1-OHP) levels, and parameters of semen quality were determined. Results: TO workers had significantly higher ambient PAHs exposure (3,436.1 +/- 3,411.0 vs. 1,123.1 +/- 1,829.3 ng/m(3), P < 0.01), urinary 1-OHP levels (207.8 +/- 176.4 vs. 54.0 +/- 44.8 mu g/g, P < 0.001), frequency of oligospermia (18.8 vs. 0%, P < 0.05), and morphological abnormality in sperm (32.3 vs. 14.6%, P < 0.01) than SO workers. Among cigarette smokers, TO workers had significantly higher rates of DNA denaturation in spermatozoa (alpha T) and percentage of sperm with increased DNA denaturation (COMP alpha T) levels than SO workers (246.2 +/- 49.5 vs. 198.1 +/- 30.3 for alpha T; 34.8 +/- 14.4 vs. 19.3 +/- 13.9% for COMP alpha T, P < 0.05). There was a positive correlation between urinary 1-OHP and alpha T, COMP alpha T, and abnormal sperm morphology and a tenfold increase in urinary 1-OHP associated with a 2.35-fold increase in alpha T, as well as a 1.07-fold increase in percentage of sperm with abnormal morphology. Conclusions: A potential risk of sperm dysfunction should be considered for workers occupationally exposed to high levels of PAHs. Cigarette smoking may aggravate this risk. Urinary 1-OHP can be used as a biomarker predicting sperm dysfunction.