Glia Contribute to the Purinergic Modulation of Inspiratory Rhythm-Generating Networks
Glia Contribute to the Purinergic Modulation of Inspiratory Rhythm-Generating Networks
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DOI：
10.1523/jneurosci.6027-09.2010
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发表时间：
2010-03-17
影响因子：
5.3
通讯作者：
Funk, Gregory D.
中科院分区：
文献类型：
--
作者：
Huxtable, Adrianne G.;Zwicker, Jennifer D.;Funk, Gregory D.
神经胶质通过释放神经递质来调节神经元的活动，这一过程被称为神经胶质传递。这个过程在控制运动行为背后的神经元网络活动中的作用尚不清楚。ATP在神经胶质传递中具有突出的特征;它还通过可能包括前Botzinger复合体（preBotC）神经网络（对呼吸至关重要的脑干中心）的兴奋的机制来促进由低氧引起的体内平衡性反应。因此，我们在体外抑制神经胶质细胞在节律性活动吸气网络中的功能，以确定神经胶质细胞是否有助于preBotC ATP敏感性。神经胶质毒素显着降低preBotC对ATP的反应，而不是其他调节剂。此外，由于preBotC神经胶质细胞对ATP的反应是增加细胞内Ca 2+和谷氨酸的释放，我们得出结论，神经胶质细胞有助于preBotC网络的ATP敏感性，并可能是缺氧性反应。数据揭示了神经胶质细胞在脑干运动网络信号处理中的作用，这可能与整个神经轴的类似网络有关。
Glia modulate neuronal activity by releasing transmitters in a process called gliotransmission. The role of this process in controlling the activity of neuronal networks underlying motor behavior is unknown. ATP features prominently in gliotransmission; it also contributes to the homeostatic ventilatory response evoked by low oxygen through mechanisms that likely include excitation of preBotzinger complex (preBotC) neural networks, brainstem centers critical for breathing. We therefore inhibited glial function in rhythmically active inspiratory networks in vitro to determine whether glia contribute to preBotC ATP sensitivity. Glial toxins markedly reduced preBotC responses to ATP, but not other modulators. Furthermore, since preBotC glia responded to ATP with increased intracellular Ca2+ and glutamate release, we conclude that glia contribute to the ATP sensitivity of preBotC networks, and possibly the hypoxic ventilatory response. Data reveal a role for glia in signal processing within brainstem motor networks that may be relevant to similar networks throughout the neuraxis.