Global Transcriptome Analysis of the Tentacle of the Jellyfish Cyanea capillata Using Deep Sequencing and Expressed Sequence Tags: Insight into the Toxin- and Degenerative Disease-Related Transcripts.
Global Transcriptome Analysis of the Tentacle of the Jellyfish Cyanea capillata Using Deep Sequencing and Expressed Sequence Tags: Insight into the Toxin- and Degenerative Disease-Related Transcripts.
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使用深度测序和表达序列标签对水母 Cyanea capillata 触手进行全局转录组分析：深入了解毒素和退行性疾病相关转录本
DOI：
10.1371/journal.pone.0142680
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Liu G;Zhou Y;Liu D;Wang Q;Ruan Z;He Q;Zhang L
研究背景水母含有多种毒素和生物活性成分。然而，大规模鉴定新的毒素和生物活性成分从水母一直受到传统的分离和纯化方法的效率低。结果我们对水母Cyanea capillata的触手组织进行了从头转录组测序。获得总共51，304，108个读段并组装成50，536个单基因。其中，21，357个unigenes在公共数据库中有同源物，但由于有限的序列信息和物种特异性的新序列，其余unigenes没有显著的匹配。单基因的功能注释也揭示了C.毛状的本研究的主要目的是鉴定推定的毒素转录本。正如预期的那样，我们筛选了许多编码与几个众所周知的毒素家族相似的蛋白质的转录本，包括磷脂酶、金属蛋白酶、丝氨酸蛋白酶和丝氨酸蛋白酶抑制剂。此外，一些转录本还类似于具有潜在毒性活性的分子，包括具有溶血活性的刺胞动物CfTX样毒素、plancitoxin-1、毒液毒素样肽-6、组胺释放因子、脑啡肽酶、二肽基肽酶4、血管内皮生长因子A、血管紧张素转化酶样和内皮素转化酶1样蛋白。这些分子中的大多数以前在水母中没有报道过。有趣的是，我们还表征了许多转录物与几种退行性疾病相关的蛋白质的相似性，包括亨廷顿病，阿尔茨海默病和帕金森病。这是第一次在水母中描述退化性疾病相关基因。结论获得了一个分类准确、注释完整的C. capillata tennis，这将是一个重要的和有价值的资源，进一步了解水母在分子水平上和信息的基础分子机制的水母蜇。本研究的结果也可用于不同水母物种之间的基因表达谱的比较研究。
Background Jellyfish contain diverse toxins and other bioactive components. However, large-scale identification of novel toxins and bioactive components from jellyfish has been hampered by the low efficiency of traditional isolation and purification methods. Results We performed de novo transcriptome sequencing of the tentacle tissue of the jellyfish Cyanea capillata. A total of 51,304,108 reads were obtained and assembled into 50,536 unigenes. Of these, 21,357 unigenes had homologues in public databases, but the remaining unigenes had no significant matches due to the limited sequence information available and species-specific novel sequences. Functional annotation of the unigenes also revealed general gene expression profile characteristics in the tentacle of C. capillata. A primary goal of this study was to identify putative toxin transcripts. As expected, we screened many transcripts encoding proteins similar to several well-known toxin families including phospholipases, metalloproteases, serine proteases and serine protease inhibitors. In addition, some transcripts also resembled molecules with potential toxic activities, including cnidarian CfTX-like toxins with hemolytic activity, plancitoxin-1, venom toxin-like peptide-6, histamine-releasing factor, neprilysin, dipeptidyl peptidase 4, vascular endothelial growth factor A, angiotensin-converting enzyme-like and endothelin-converting enzyme 1-like proteins. Most of these molecules have not been previously reported in jellyfish. Interestingly, we also characterized a number of transcripts with similarities to proteins relevant to several degenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s diseases. This is the first description of degenerative disease-associated genes in jellyfish. Conclusion We obtained a well-categorized and annotated transcriptome of C. capillata tentacle that will be an important and valuable resource for further understanding of jellyfish at the molecular level and information on the underlying molecular mechanisms of jellyfish stinging. The findings of this study may also be used in comparative studies of gene expression profiling among different jellyfish species.