An improved experimental scheme to measure self-diffusion coefficients of biomolecules with an advantageous use of radiation damping
An improved experimental scheme to measure self-diffusion coefficients of biomolecules with an advantageous use of radiation damping
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DOI：
10.1016/s0009-2614(99)00143-8
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发表时间：
1999-03-19
影响因子：
2.8
通讯作者：
Cosman, M
中科院分区：
文献类型：
--
作者：
Krishnan, VV;Thornton, KH;Cosman, M
提出了一种改进的核磁共振 (NMR) 实验方案，使用脉冲磁场梯度测量 95% H2O 中生物分子的自扩散系数。水的辐射阻尼对高场NMR中的光谱质量产生有害影响，有利地用于减少水信号。新序列的增强功能被命名为 BPP-SED，它是 BPP-LED（双极梯度脉冲对纵向涡流延迟）和 SED（选择性回波相移）的新型组合，通过测量有限体积（250 μl）中稀释蛋白质样品（0.5 mM）的自扩散系数来证明。 (C) 1999 Elsevier Science B.V. 保留所有权利。
An improved nuclear magnetic resonance (NMR) experimental scheme to measure self-diffusion coefficients of biomolecules in 95% H2O using pulsed-magnetic-field gradients is presented. The radiation damping of water, which deleteriously effects the quality of the spectra in high-field NMR, is advantageously used in reducing the water signal. The enhancements of the new sequence, designated BPP-SED, a novel combination of BPP-LED (bipolar-gradient-pulse pair longitudinal eddy current delay) and SED (selective echo dephasing) is demonstrated by measuring self-diffusion coefficients of dilute protein samples (0.5 mM) in limited volumes (250 mu l). (C) 1999 Elsevier Science B.V. All rights reserved.