Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
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DOI：
10.3389/fmats.2021.756055
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发表时间：
2021-11
期刊：
影响因子：
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通讯作者：
J. Dugan;C. Colominas;A. García-Granada;F. Claeyssens
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文献类型：
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作者：
J. Dugan;C. Colominas;A. García-Granada;F. Claeyssens
本研究报告了一种通过聚（低聚乙二醇甲基丙烯酸酯）（pOEGMA）表面功能化和随后的激光消融产生细胞粘附轨迹来空间控制神经元粘附到类金刚石碳（DLC）上的途径。DLC可以作为坚韧和低摩擦涂层沉积在可植入设备和外科器械上，并且具有用作生物材料的有利特性。pOEGMA表面涂层使DLC表面光滑，激光烧蚀在表面上产生石墨化轨迹。表面涂有层粘连蛋白，层粘连蛋白优先粘附在消融轨迹上。研究了图案化表面的神经元细胞生长，NG 108 -15细胞用于短期培养，大鼠神经干细胞用于长期培养。细胞最初高度选择性地粘附到消融轨迹，而长期细胞培养显示表面的更均匀的细胞覆盖。
This study reports a route to spatial control of neuronal adhesion onto Diamond-Like Carbon (DLC) by surface functionalisation by poly (oligo-ethyleneglycol methacrylate) (pOEGMA) and consequent laser ablation to produce cell adhesive tracks. DLC can be deposited as a tough and low friction coating on implantable devices and surgical instruments and has favourable properties for use as a biomaterial. The pOEGMA surface coating renders the DLC surface antifouling and the laser ablation creates graphitised tracks on the surface. The surfaces were coated with laminin, which adhered preferentially to the ablation tracks. The patterned surfaces were investigated for neuronal cell growth with NG108-15 cells for short term culture and rat neural stem cells for longer term culture. The cells initially adhered highly selectively to the ablation tracks while longer term cell culture revealed a more uniform cell coverage of the surface.