Divergence between C-melo and African Cucumis Species Identified by Chromosome Painting and rDNA Distribution Pattern
Divergence between C-melo and African Cucumis Species Identified by Chromosome Painting and rDNA Distribution Pattern
复制标题
通过染色体绘制和 rDNA 分布模式鉴定甜瓜和非洲黄瓜物种之间的差异
DOI：
10.1159/000453520
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发表时间：
2016-01-01
影响因子：
1.7
通讯作者：
Han, Yonghua
中科院分区：
文献类型：
--
作者：
Li, Kunpeng;Wang, Huaisong;Han, Yonghua
5S 和 45S rDNA 位点是有用的染色体标志，可以提供有关核型进化和物种相互关系的宝贵信息。在本研究中，我们采用荧光原位杂交 (FISH) 来确定 8 个二倍体黄瓜物种中 5S 和 45S rDNA 位点的数量和染色体位置。将两种对甜瓜携带 rDNA 的染色体特异的寡核苷酸绘制探针与其他黄瓜物种杂交，以研究黄瓜物种中携带 rDNA 的染色体之间的同源性。分析的二倍体物种显示出 3 种类型的 rDNA 分布模式，这为甜瓜和野生二倍体非洲黄瓜物种之间的差异提供了明确的细胞遗传学证据。目前的结果不仅显示了黄瓜属物种之间的相互关系，而且 rDNA FISH 模式也可以用作细胞学标记来区分密切相关的物种。这些数据将有助于育种者从各种野生品种中选择最合适的品种来改良栽培甜瓜。
The 5S and 45S rDNA sites are useful chromosome landmarks and can provide valuable information about karyotype evolution and species interrelationships. In this study, we employed fluorescence in situ hybridization (FISH) to determine the number and chromosomal location of 5S and 45S rDNA loci in 8 diploid Cucumis species. Two oligonucleotide painting probes specific for the rDNA-bearing chromosomes in C. melo were hybridized to other Cucumis species in order to investigate the homeologies among the rDNA-carrying chromosomes in Cucumis species. The analyzed diploid species showed 3 types of rDNA distribution patterns, which provided clear cytogenetic evidence on the divergence between C. melo and wild diploid African Cucumis species. The present results not only show species interrelationships in the genus Cucumis, but the rDNA FISH patterns can also be used as cytological markers for the discrimination of closely related species. The data will be helpful for breeders to choose the most suitable species from various wild species for improvement of cultivated melon.